...lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a...typically express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate ...with a single-vector system). Tips for using this plasmid Cas9 expression is typically cytotoxic, which makes...requested lentivirus and AAV of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9... Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that...lentiCas9-blast lentivirus from Addgene, you could use it in conjunction with gRNA lentiviruses easily made...Viral Service and began delivering ready-to-use lentivirus and adeno-associated virus (AAV) to scientists...

... and lentiviral preps! Download the green AAV background.       Some of our lentiviral plasmids include...envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background.     ...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...      We also have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries...

...transfection of single plasmids vs pooled plasmids; for multiple edits, single plasmids are generally more...Sakuma et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses...transfected on a single plasmid. This is versus 2.4% of cells transfected with a pool of plasmids that contained...expressing the Cpf1 multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the...sites. This eliminates bulk from crRNA expression plasmids resulting in smaller, more streamlined vectors... multiple expression platforms: transfection, lentiviral transduction, and AAV transduction.  In most ...contained one crRNA per plasmid plus a Cpf1 expression vector.  See the graph in figure 2 for a comparison of...

...or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus As some of the most popular...accelerate your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many...collection. We’ll provide a brief rundown of what lentiviral and AAV preps are available below, but you can... popular lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral...Viral Service with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators...with viruses including advice on how to titer lentiviral preps, troubleshoot viral transductions, and ...Virus in Your Research: A Primer for Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...crux of the system is a set of heat-inducible lentiviral CAR constructs, the most basic of which is depicted...

...diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries ...digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from...from the pAWp28 storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA...recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library." Nature biotechnology...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...

...CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools...CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery...toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression... Cas9. Recently, Baisong Lu’s lab developed a lentiviral capsid-based bionanoparticle system for packaging...Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang’s ...RESCUE. Read the paper in Science Find RESCUE plasmids If you have a new CRISPR tool you’ve recently...

...considered safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing and nondividing...Excisable Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but ...into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the ...reprogramming factors. Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial... generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another...the dose of plasmid the cells receive over the whole reprogramming period and the plasmid will get diluted...larger plasmids have lower rates of transfection. Minicircles: Minicircles are like mini-plasmids. They...

... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...resources on the Addgene blog Plasmid 101: Inducible Promoters Plasmid 101: Repressible Promoters CRISPR...knock out. Looking for some permanent knock out plasmids? Check out our Cre vector and Murine LoxP vector...of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn it on...transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector...promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector...Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging ...

...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ...Empty Backbone) Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction...with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg ...

...300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed... best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids from ...through to the plasmid search results: More than 200 different GCaMP containing plasmids are available...search tool lets you access plasmids from journal articles. From paper to plasmid Authors regularly indicate... to include specific plasmid ID numbers. If you know the ID number for a plasmid, your straightforward...page to see the plasmids deposited by our own CSO. But what if you don’t have plasmid ID numbers, or you... Not every plasmid search will start from a publication. You may have an idea for a plasmid that would...

... top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many ...requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope...top requested plasmid of 2018! Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and ...the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin... Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit...

...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything...very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's...

...efficiency. Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...Packaging and Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...: Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome repair by...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide...

...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...within the lentiviral 3’ long terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...license. In this iteration, the size of the lentiviral insert was minimized to reduce recombination ...III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These optimizations... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...

... LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable. General...in trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging cell...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to... within both the 5' and 3' LTRs of retro/lentiviral plasmids. U5; Unique 5'; region at the 5' end of the...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest..., which facilitate integration of the transfer plasmid sequences into the host genome. Typically, it is...

...don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes a plasmid, the genes on the... the plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent...This can be done with lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to... You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations... Read all of our Plasmids 101 blog posts Check out all of our blog posts on plasmid cloning Resources...comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the environment...membrane so the bacteria can easily uptake your plasmid of interest. Scientists can also use electroporation...

...brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral...wider diffusion is required. On the other hand, lentiviral vectors are superior packaging vectors for larger...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G...injection. Find more details about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector...1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious units per... share ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral...

...on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References... the lentiviruses (like HIV-1) — that have been developed into lentiviral vectors. Lentiviruses are sometimes... 1: Gamma-retrovirus genome (left) and an HIV lentiviral genome (right.)  HIV seems an unlikely candidate...Addgenie Rebecca Hutcheson for her assistance with lentiviral replication.  Resources and References More ...PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems ...

...mutation Improves plasmid yield F Host does (F') or does not (F-) contain the fertility plasmid. A low copy-number...and general DNA repair Reduces plasmid recombination, increases plasmid stability recBCD Exonuclease...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected the optimal promoter for...labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count...whether it is appropriate for propogating your plasmid or carrying out your experiment. History of E. ...major improvements such as those that increase plasmid yield and/or DNA quality. Table 1 below outlines...