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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31 results for: lin tian

Showing: 21 - 31 of 31 results

  1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...features that make zebrafish useful, I’ll outline two recent lineage tracing approaches that led to important...screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated...fish lines have been developed to enable high-resolution mapping of the brain by uniquely labeling neurons...zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources and highlight...of the zebrafish genome was published in 2013, revealing that about 70% of human genes have a counterpart...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...

  2. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...quickly along regions calling for abundant, charged tRNAs but will stall at regions calling for low abundance...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...databases showing various organisms’ codon preferences online). Even if the gene is translated, it may not function...synthesis companies such as IDT and GenScript host online codon optimization tools. Keep in mind that, just...and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression is enhanced...PubMed Central PMCID: PMC2970903. 6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation...

  3. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...lesioning methods -  they may be the key to further unraveling the mysteries of behavior. We’ve previously ...to the neuronal networks involved in directly controlling muscle activity and coordinating movement, several...stress response circuit by integrating neuronal signalling and triggering release of the stress hormone ...regions such as the nucleus accumbens (NAc), which is linked to the reward circuit, and the medial prefrontal...optogenetics to support these existing findings by controlling neurons in the mPFC with light. Activation of...24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher. "Refining deep brain stimulation to emulate...

  4. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...Typically, regularly spaced denticles (bristles) outline the segments of the Drosophila embryo. In flies... was reading. SHH is involved in the hedgehog signalling pathway, which is a key regulator of all bilateral...1189. PubMed PMID:1905980. Nüsslein-Volhard, Christiane, and Eric Wieschaus. "Mutations affecting segment...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...Pennsylvania State University. Imagine you are dealing with a defective gene, Xm, the sequence of which...PubMed Central PMCID: PMC5726555. 4. Xie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure 2) and...control (WT). Vinculin is a loading control. 3. In terms of making the monoclonal line: I had never...cancers) in A549 cells (a human lung cancer cell line), armed only with viruses obtained through Addgene...the edits without any reagent-grade endangered Martian chicory root, but considering this is a needle ...cells were then used to generate a monoclonal cell lines by limiting dilution. The rest of the cells were...indicated multiplicity of infection (MOI). Anti-vinculin Western blotting is a loading control. Pro-Tips... Figure 2: Cas9 expression in monoclonal cell lines generated from A549 cells transduced with lentiCas9...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... in the tissue/which cell type? Location (3): Traveling from the original location - Axonal Transport ...mouse brain (4). An example from the cortex is outlined in the table below. Cell Type Cortex Optimal... the best AAV for your particular experiment! Traveling from the Original Location - Axonal Transport ... (4). Otherwise, using a specific system for controlling gene expression such as Cre-lox, may be sufficient...using Cre-lox, this new method could allow for labeling of neurons showing a very specific phenotype. ...PubMed PMID: 18414476. 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory...variety of constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...Crossref, https://doi.org/10.1002/tpg2.20312. Brychcy, Merlin, et al. “AspFlex: Molecular Tools to Study Gene... Multiplex Editing: One‐shot Generation of 8× Nicotiana Benthamiana and 12× Arabidopsis Mutants.” The ...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add...multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden Gate and ... overhangs. These overhangs can be customized to link together multiple fragments, allowing ordered assembly...enzyme, the unique flanking overhang sequences can link together to allow for ordered assembly into a destination...overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and drive ordered assembly into...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...what implications their signaling is likely to have). Anterograde tracing outlines neurons from their cell...areas. It is desirable for tracing compounds to outline neurons in the direction of information flow in...terminals of their axons; while retrograde tracing outlines neurons in the opposite direction, from the terminals...so spread across several synaptic connections (Ugolini et al., 1989). When using these viruses to mark...neuronal tracing has the added benefit of only labelling the subset of infected cells that also express.... Differences between AAV serotypes may be also linked to differences in viral uptake by cells and to ...identification requires both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...proteins play in cell signaling. Understanding how molecules in the Ras signaling pathway interact will...314 codon-optimized GPCR sequences for human cell line expression. In a twist on the classical TANGO assay...coli gene expression plasmids with the Cross-disciplinary Integration of Design Automation Research lab...Ras proteins are small GTPases involved in cell signaling pathways that control many cellular processes ...containing genes from a crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each...expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall, et al. Methods Mol...unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many of which ...
Showing: 21 - 31 of 31 results