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We narrowed to 33 results for: luciferase expression

Showing: 21 - 33 of 33 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ... yeast expression, worm expression, insect expression, plant expression, mouse targeting, CRISPR, TALEN... mammalian expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Description Expression Additional considerations CMV General expression mRNA Strong mammalian expression promoter...lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...some cell types. EF1a General expression mRNA Strong mammalian expression from human elongation factor ...give consistent expression regardless of cell type or physiology. SV40 General expression mRNA Mammalian...  Commonly used in expression systems for Drosophila. Polyhedrin General expression mRNA  Strong insect...Constitutive  Commonly used in expression systems for insect cells. CaMKIIa Gene expression for optogenetics mRNA...neuronal/CNS expression. Modulated by calcium and calmodulin. GAL1, 10 General expression mRNA Yeast ...

  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...the receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab...resource called PRESTO-TANGO (parallel receptorome expression and screening via transcriptional output - TANGO...preps from DREADD plasmids allow you to easily express a DREADD receptor in a specific neuronal subtype...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ... for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...cells that are expressing your gene of interest at high levels. Unlike vectors that express screenable or...want to express multiple genes in the same cell. Although it is possible to drive co-expression by using... with multiple, individual expression cassettes, having the genes expressed from the same cassette is ...designed for bicistronic expression; however, many could be easily manipulated to express more than two genes...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...multicistronic vectors? Detecting cells that are expressing your gene, especially if you are studying a novel...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression Level of Gene...as the expression level of Gene X in cells treated with Plasmid A is similar to the expression level of...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...decreased expression of Gene X. Ergo the positive control(s) should decrease the expression of Gene X....into the cells and the ability of the cells to express the protein.   In analysis of the result in Figure...Figure 1, an internal control, such as the GFP-expressing Plasmid B, could demonstrate whether the cells...cells were transfected successfully and expressing the protein. For example, fluorescence microscopy images...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology arm ...-injection of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP... then mixed and transfected along with a Cas9 expression vector. They can also be introduced directly ...directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging... template for inducible alteration of protein expression levels. CRISPR tagging systems offer users an... tag endogenous proteins for studying protein expression, localization, and protein-protein interactions...

  7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...same strain. MazF expression is under control of the AOX1 promoter whose expression is induced by methanol...that are used to drive high protein expression in vivo. Expression plasmids are often lost without plasmid... and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters...hok/sok system to create strains that can stably express antigens in sufficient quantities to generate an...plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene."...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane ASAP5...Figure 2), the authors developed a cost-effective expression and labeling approach using these constructs ...protein of interest can be inserted. B) Protein expression and labeling procedure. Adapted from Smith et..., and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful not only for future...Janicot and Maziarz et al., 2024). Each plasmid expresses all the components of the biosensor in optimal... a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...bacterial genome, and inserted it into E. coli to express and then test for any proteins that could cut RNA..., when they looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition...RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using Cas7-11   Use ...

  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 toxic proteins... gRNAs to target the HIV-1 5’ and 3’ LTRs and expressed them along with Cas9 in the T-cell line 2D10. ...of the LTRs joined together. Clonal Cas9/gRNA-expressing cell populations were also immune to HIV-1 reinfection...cycle progression, or apoptosis with Cas9/gRNA expression. A pooled analysis found no evidence of Cas9 .... For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, ...preventing re-infection would require sustained expression of Cas9/gRNAs, which could result in higher rates...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we added ...added curation fields for purpose, promoters, expression types, and more, all of which help a researcher understand... Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids Blogs Origin of ...
Showing: 21 - 33 of 33 results