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We narrowed to 41 results for: mcherry reporter

Showing: 21 - 40 of 41 results

  1. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are...Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized ...panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate ...Image from Diessl et al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed in ... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations... commonly used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus...GFP. They then tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells ...tested in retinal electroporation studies, DsRed reporter expression was induced in ~76% of GFP+ cells, ...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry). They additionally discovered...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The ...last month, Robert Campbell’s lab added two new mCherry variants to the repository. These variants can ...ONE paper for more on the directed evolution of mCherry and the spectral properties of various RFPs. For...reagents shared via Addgene. Narayan S, et al. Cell Reports. 2017. PubMed PMID: 28813671 Listen to the podcast...Listen to Our Podcast Segment on PhoCl LSSmCherry1 & RDSmCherry1: Engineering and directed evolution of...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...), tagRFP (pKa = 3.1) and mCherry (pKa = 3.8). We have observed that mCherry (and mTurquoise2, not shown...interest with an additional protein module that can report on location. This approach enables the study of...9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion in ...

  6. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...followed by an internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance..., Bernach plans to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins...colonizer, labelled with a constitutively fluorescent reporter can also be used to track how one population responds...using TIMER and an unstable fluorescent protein reporter under the control of promoters that control cell... the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously changes from...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...artificial leaf, Bernach will use the CUSPER bioreporter such that fluorescent proteins expression is ...

  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...along with a reporter, such as a fluorescence gene or a resistance gene. These reporters allow you to ... using IRES elements. Further, scientists have reported lower expression of the downstream cistron due...eukaryotic cells, and, although some scientists report close to 100% cleavage with some of these peptides...that already has the multicistronic element and reporter cloned in. In these plasmids you would simply ... 2A element (depending on the placement of the reporter gene). Addgene's collection offers a variety of...Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra ...multicistronic element(s), or you could replace one of the reporter genes above with your gene or genes of interest...

  8. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...second. The first successful irreversible PA-FP reported was PA-GFP. PA-GFP was derived from Jellyfish ...mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / A58T / K69N / L84F / N99K / S148L / I165V...UV-Violet or blue Green/ Red 4,500 High 553/ 573 PA-mCherries Monomer UV-Violet Dark/ Red >3,000 Medium 570/.... 2.Subach, Fedor V., et al. "Photoactivatable mCherry for high-resolution two-color fluorescence microscopy...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...p2A-ferritin-p2A-mCherry Mammalian Expression 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...and named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was...Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...

  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He says, these new...

  11. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...produce complex fluorescent reporter constructs. These fluorescent reporters can be used to detect structure...protein sequences to develop diverse fluorescent reporters is a useful application of high-throughput cloning...the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm...

  12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring... which cannot form heterodimers (i.e. EGFP and mCherry). These simple strategies can help you make better...better fluorescent fusion protein reporters for your studies and greatly lower your chances of finding ...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...

  14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators... open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow cycling (open for minutes) ...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...developed monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...

  16. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...is to fuse them with a fluorescent reporter, such as GFP or mCherry, through genetic modification. As ...combination with that reporter, you can infer its presence through detecting the reporter's fluorescence. Another...combination of antibody-fluorophores, fluorescent reporters, and/or dye stains used in your flow cytometry...mentioned previously, you can also use fluorescent reporters, such as GFP, and fluorophore-conjugated antibodies...compare the excitation/emission spectra of your reporter with the ones from your fluorophore labels and...encodes for eGFP, which serves as a transduction reporter. Note that eGFP is not fused to CD45, but is separately...for having a separately expressed transduction reporter is that not every transduced cell will necessarily...

  17. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression of dCas9...astrocytes, and oligodendrocytes, provided that reporter genes specific to these cell types are used. The...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... are named for soup ingredients! From pSOUP to mCherry, satisfying ingredients reagents permeate the molecular...growth as RNA biology, we are looking forward to reports on the next additions to aptamer soup! References...
Showing: 21 - 40 of 41 results