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We narrowed to 192 results for: mica

Showing: 21 - 40 of 192 results
  1. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...processes that are influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM...prescribed region of DNA termed the “register”. Each chemical input to an RSM drives expression of a distinct...to record all permuted substrings of a set of chemicals. Specific examples of arrangements that should... record all permuted substrings of up to seven chemical inputs are given in Table S2 of our paper. The...
  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...by the use of AAVs? 1) Combined functional and anatomical mapping of circuits In our lab, we use AAV-DREADD...these complexes are so robustly expressed that anatomical assessments can be easily performed too. While...identified this feature of AAVs as a new tool for neuroanatomical tracing (11). With the ability to produce neuron... Floris G., et al. "A fourth generation of neuroanatomical tracing techniques: exploiting the offspring...
  3. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...themselves from attack. It is a region of incessant chemical activity, and consists of other cell types, especially...attack the cancer cells are also suppressed by chemical signals such as IL-8, IFNγ, and colony stimulating...metastasis or eliminate the probability of a relapse. Chemical and radiation therapies cause a transient shutdown...identities based on their level of progression, anatomical location, characteristic (solid localized tumors...
  4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... these specimens has offered illumination on biochemical processes that are crucial in the world of biological...interest. Fluorescent dyes and ligands vary in their chemical structure, leading to differences in their excitation...Nozaki, N., & Kimura, H. (2014). Evaluation of chemical fluorescent dyes as a protein conjugation partner... of ligands with G protein coupled receptors. Biochimica et Biophysica Acta - Biomembranes, 1838(1), 15...
  5. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ... is necessary for bacterial transformation of chemically and electrocompetent cells is minimal (picogram...nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used during the purification...addition to being free of any nucleic acid and chemical contaminants, these DNA samples must also be entirely...
  6. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...side of things, the authors optigenetically and chemically excited a class of enteroendocrine cells within...both of these stimulations. The best part? The chemical stimulation was sucrose…..sounds like the gateway...fiber-based optoelectronics for neural interfaces. Chemical Society Reviews, 48(6), 1826–1852. https://doi.org...
  7. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy by: Susanna Stroik...many of these applications have lagged. Fixation, chemical staining, and light exposure can negatively affect...Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy...
  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo wants to simplify...simplify access to academic resources among economically disadvantaged populations. Roo is the co-founder...resources that can help their children thrive academically. Rose is the co-founder of STEAMid.     Additional...
  9. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... are made of chemicals, which have an unfortunate tendency to react with other chemicals and biological... fluorophores, which work not by reacting to a chemical, but by emitting a specific wavelength of light...
  10. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    Published
    June 26, 2018, 1:33 p.m.
    ... to investigate the use of a common laboratory chemical, guanidine hydrochloride, as a potential treatment...the specific molecular features that allow such “chemical rescue” in vivo. Engineering the human disease...Dr. McMurray hopes that by demonstrating GdnHCl chemical rescue of a variety of disease-linked Arg-mutant...
  11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...engineered microbes, used coffee grounds could be economically repurposed as starting material for the bioproduction... FLINC biosensors for live-cell imaging of biochemical activity Article contributed by Angela Holmes... live-cell imaging of the dynamic changes in biochemical activity within the cell. Their Fluorescence ...
  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ..., & Zoltowski, B. D. (2021). Cryptochromes: Photochemical and structural insight into magnetoreception...Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification of magnetic field effects relevant...Persistent Diradical Emitter. Journal of the American Chemical Society, 145(25), 13615–13622. https://doi.org...
  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate...  The first successful attempt to render fixed anatomical preparations transparent was done by Walter Spalteholtz...that also allows antibody penetration for immunohistochemical labeling in whole tissue (15).  CLARITY is...
  14. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...cell membrane but inside the cell. Due to their chemical nature, antibodies cannot penetrate the cell membrane...intracellular labeling with antibodies requires chemically pre-treating the cells to allow for entry of ...intracellular labeling protocol is necessary, requiring chemical treatment of the cells after surface labeling....
  15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...developed a version of Tetbow that incorporates chemical tags instead of XFP to optimize it for tissue ...architecture of a fluorescent neuron is visible. Chemical tags are more resistant to tissue clearing methods...neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (2018): e40350. PubMed PMID: 30454553...
  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...effects on cell growth. The C321.ΔA strain was genomically recoded to eliminate all 321 amber stop codons...Pirman, Natasha L., et al. "A flexible codon in genomically recoded Escherichia coli permits programmable...PMCID: PMC4566969. 7. Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions...
  17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...mobility than the static microscopy images of chemically coloured lymphoid organs did in the past. How...movements. By using cells labelled by fluorescent chemicals or by other fluorescent proteins, labs were also...(proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins (...
  18. Purple Plants that Report Pollutants

    Type
    Blog Post
    Published
    March 3, 2026, 2:14 p.m.
    ...problematic ligands, crops could report the presence of chemical contaminants in their fields. The authors call... system would be best suited for detection of chemicals that readily diffuse through plant tissue. Those...
Showing: 21 - 40 of 192 results