We narrowed to 26 results for: monomeric gfp

1. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...LwaCas13a, which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. ...

2. The Fluorescent Vegetables in Aptamer Soup

   Type

   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone...and may be a better choice for imaging than the monomer. Broccoli has been developed into a split aptamer...

3. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on...and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the...enabled scientists to engineer a wide variety of GFPs with diversity in fluorescence brightness, intensity...sensitivity at lower expression levels. APEX2 is a monomeric, 27 kD genetic tag which can be fused to proteins...Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists to validate the efficacy...

4. Hot Biosensors 2022: Year-End Roundup

   Type

   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...genetically encoded calcium indicator using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720...

5. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

   Type

   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ...while the emission maximum is 675 nm. mCarmine is monomeric, 4-5 fold brighter than mNeptune684, and has a...real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence...involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy or...

6. FPbase: A new community-editable fluorescent protein database

   Type

   Blog Post

   Published

   May 16, 2018, 1 p.m.

   ...multi-parametric search queries.  Looking for a bright, monomeric FP published in the last few years with an emission...relating to that specific protein (for instance: EGFP).  Users can “favorite” certain proteins, or create...