We narrowed to 99 results for: mouse c rel

...using a mouse model for a human neurological disease (Sinnamon et al., 2020). They introduced a mouse MECP2...10.1126/science.1206593 Powers S, Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein...al., 2007). They accomplished this using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted... ADAR2, into the hippocampus of a Rett Syndrome mouse model using an adeno-associated viral (AAV) vector... A (2007) Reversal of Neurological Defects in a Mouse Model of Rett Syndrome. Science 315:1143–1147 . ....2020.107878 Sinnett SE, Hector RD, Gadalla KKE, Heindel C, Chen D, Zaric V, Bailey MES, Cobb SR, Gray SJ (2017... expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene and share additional...

...raw %}{"no_follow":false,"open_in_new_tab":true,"rel":"noopener","sponsored":false,"url":{"content_id"...types. Most enhancers were derived from the human, mouse, and macaque genomes, and are designated by a letter...(2025). A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types....elements drive subclass-selective expression from mouse to primate neocortex. Cell Reports, 34(13), 108754...https://doi.org/10.1016/j.cell.2025.05.002 Hunker, A. C., Wirthlin, M. E., Gill, G., Johansen, N. J., Hooper...Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., . . . Tasic, B. (2021). Enhancer viruses for combinatorial...Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli, C., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., ...

...before working with mouse models, several common techniques used to create engineered mouse models at embryonic...understand mammalian traits and genetically engineered mouse models provide researchers with useful and adaptable...preclinical research. For scientists new to using mouse models, the possibilities may seem endless - and...editing techniques. Inbred and outbred strains Mouse strains used in research can fall under one of two...common genetic background, which means that each mouse is genetically identical and all mice of the strain... effect related to an experimental manipulation. Common inbred strains include C57BL/6, Balb/c, and 129...inserted at a specific site. To generate a transgenic mouse, microinjection of DNA or infection with a viral...

...should I pick? Do I go for the rabbit or the mouse? Is mouse IgG1 better or IgG2a? Will my choice affect... anti-mouse antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody... For example, an anti-mouse IgG2a secondary would be a better choice for a mouse monoclonal IgG2a than...species-on-species, i.e. using a mouse derived primary antibody to stain a target protein in mouse tissues, causes a ...are using mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed...antibody. Some are subclass specific, like an anti-mouse IgG2a antibody. When using a monoclonal antibody...than a general anti-mouse IgG. In a broadly specific secondary antibody only a fraction of the antibody ...

...small molecules targeting SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...disease are unclear. Dr. Di Schiavi plans on using C. elegans as a model to identify SMN1 interacting partners...effects of smn1 disruption. As part of his work in C. elegans, Dr. Di Schiavi will use Addgene plasmids...these genes, CRISPR to disrupt their function, and C. elegans overexpression plasmids to upregulate their...research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic review of incidence...heart health. These are amazing advances that will surely save thousands if not millions of lives, but many...

...Zhi Ying Chen, Robert C. Robbins, Mark A. Kay, Michael T. Longaker, and Joseph C. Wu. "A nonviral minicircle... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-... mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves making chimeras by injecting...functional germ cells. The highest stringency test for mouse iPSCs, tetraploid complementation, involves injecting...measuring their ability to direct the development of a mouse. Since many of these functional tests aren’t possible...Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon." Nature Methods...

...is a recombinant version of the original V5 tag mouse monoclonal antibody, which recognizes a short, linear...applications.  IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Optimized mScarlet-based tags for live imaging in C. elegans CRISPR/Cas9 for gene disruption in M. abscessus...tagging proteins, as it can be fused to the N- or C-terminus (or even in the middle of proteins) and performs.... E., Young, D. F., Goswami, K. K., & Russell, W. C. (1987). Isolation and characterization of monoclonal...39406745. Optimizing mScarlet tags for live imaging in C. elegans By Alyssa Neuhaus Red fluorescent proteins...maturation rate limits its use in systems like the C. elegans embryo, where developmental processes happen...

... Sellnow, R. C., Boye, S. L., Coberly, B., Bennett, A., Agbandje-McKenna, M., Sortwell, C. E., Hauswirth...strains of the same species. What works well in a mouse may not work as well in a rat or non-human primate...Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLOS ONE, 8(9), e76310. https://doi.org/10.1371... vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research...Backer, M. W. A., Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization...Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe..., J. Y., Han, J. H., Lee, H., Han, Y. E., Rah, J. C., & Park, H. (2020). Labeling dual presynaptic inputs...

...transdifferentiation in vitro where treatment of mouse fibroblasts with 5-azacytidine led to their conversion...2000, Ferber et al. showed for the first time that mouse liver cells could be transdifferentiated in vivo...Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined...PMC2758053. 8. Okita, K., et al., Generation of mouse induced pluripotent stem cells without viral vectors...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming ...22645348. PubMed Central PMCID: PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth...

...Chain Fragment Variables (scFvs)Avoiding the Mouse on Mouse Mess in IHC Resources on Addgene.org  Browse...The modularity of antibodies means that it is relatively easy to physically divide up the different regions...fragments intended for use as primary antibodies are relatively uncommon. Though, if you want to use Fab fragments...features labeled. V = Variable domain. L = Light chain. C = Constant domain. H = Heavy chain. Created with biorender.com...

.... K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q.,...representative micrograph (C), particle orientation distribution and Fourier-shell correlation curve (D), and the... of delivery could be used to partially correct mouse models of genetic blindness (An et al., 2024) — ...could silence the expression of prion protein in mouse brains for at least six months. Unlike genome editing... module (SpyTag-GFP nanobody) assembled on beads. C) Micrograph of H1.8-GFP nucleosomes collected with...Protein samples protected from AWI by helical LEAs. C-E) cryo-EM single-particle analysis of PRC2 with RvLEAMshort...tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim, C. J. (2024). Small LEA proteins as an effective air-water...

...-018-0219-4  Yu C-C (Jay), Barry NC, Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife...easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans (Damstra, et...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable.../doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide to ...they want to understand the ultrastructural relationships that influence everything from cell division...optimization and careful execution, but they cannot be entirely eliminated. It is recommended that you image samples...

...difficult to grow. Growth Temp Choose from 30 °C, 37 °C, or room temperature. Growth Strain Please... Choose from: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken), B. taurus...expression, insect expression, plant expression, mouse targeting, CRISPR, TALEN, synthetic biology, affinity...frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast)...be indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as ...

...these attributes of Cre-lox to create the Brainbow mouse neural labeling system. Brainbow-1 and -2 have been...Brainbow constructs into mice. When they imaged the mouse brains, they found many more than 3-4 colors due... of limitations. First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity...  Brainbow variants and applications beyond the mouse brain In addition to improving the Brainbow system...Sato T, van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers...melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. ...observed in a given area. In 2013, Dawen Cai, et al. released a refinement of the Brainbow technology, Brainbow...

...Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, or C base editors...transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like... a more specific A → C base editor (ACBE), they inserted the deaminase into the C-terminal domain of Cas9...pyrimidines (C ↔ T).   Base transversion Base change from purine (A or G) to pyrimidine (C or T) or vice-versa...type of base editor invented. CGBE C → G Base Editor Converts C → G by fusing a uracil DNA glycosylase..., or C, respectively, to be replaced with another base. References Chen, L., Hong, M., Luan, C., Gao... Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C., Zhu, Y., Guan, Y., Wang,...

...deposit please sign up here.        A retroviral mouse CRISPR knockout library Article contributed by Alycia... factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening ...screens in cells with few genetic tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et...this may be due to different lipid compositions of C. elegans membranes, which might beneficially influence... of ApoB-LPs has been shown to have a strong correlation with cardiovascular disease and indirect measurement...that the system is active in E. coli and closely related species, is amenable to use with other bEBP/UAS...and the system may not be adaptable to distantly related species from E. coli. This tool should serve as...

...12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor...in the primary somatosensory cortex (S1) of the mouse brain following retro-orbital AAV injection at 4...expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for single action potential-evoked...tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin...integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi, M., … Abudayyeh... Cenik, B. K., Aoi, Y., Iwanaszko, M., Howard, B. C., Morgan, M. A., Andersen, G. D., Bartom, E. T., &.../doi.org/10.1016/j.molcel.2024.11.007. Branon, T. C., Bosch, J. A., Sanchez, A. D., Udeshi, N. D., Svinkina...

...differences in chemogenetics and optogenetics in a mouse experiment. Viral delivery is the same in both cases...light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...annurev-pharmtox-010814-124803 Vlasov, K., Van Dort, C. J., & Solt, K. (2018). Optogenetics and Chemogenetics...level of control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs...throughout the body. Controlling Stimulation Some studies rely on delivering reversible or varied amounts of stimulation... of being less invasive and more flexible as it relies on designer ligands that can be simply introduced...

...well? Our collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit Fc) anti-FLAG... al. (2023). Efficient engineering of human and mouse primary cells using peptide-assisted genome editing...enabling repeat dosing. In a proof-of-concept study, mouse LY6A was delivered to brain endothelial cells in...mChe), with co-incubation with assist peptide (AP). C) Workflow for Cas-RNP-PAGE editing of primary T cells... and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this method is...

...stronger fluorescent signals upon sensing glutamate in mouse and ferret models. Furthermore, they created three...showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation ...Improved trafficking and localization through different C-terminal anchoring domains The original iGluSnFR in...adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal fusion to the PDGFR transmembrane domain ...mammalian expression pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains...domain, followed by a modified form of the cytosolic C-terminal domain of Stargazin including a terminal ...give researchers the ability to monitor glutamate release from neurons and other brain cells in vivo. Glutamate...