...Resources Review of restriction modification systems NEB's Resource describing Dam and Dcm Methylases Discovery...

...ligation mix in different bacterial strains (stbl2, NEB stable, etc.), and/or use a different temperature...

...blog High Complexity Golden Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids...

...however, there are a few popular lab strains, such as NEB Stable, JM109, XL1 Blue or XL10 Gold, that are F’...

...reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good places to look for some helpful kits...

...questions (besides Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’...

... plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality DNA that...

...bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain, like DH5α, grown...

...stability, such as some of our viral vectors, in NEB Stable cells. Resource information: This section ...

...expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies...

...viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are...

...for storage and distribution at Addgene are DH5α, NEB stable, ccdb survival, and Pir1. The majority of ...

...to your own research needs. Other companies like NEB and ATCC can provide you with additional validated...

...al., 1981). E. coli strains such as DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and...

...Cloning High Complexity Golden Gate Cloning with NEB’s DAD New MoClo basics blog ...

...Overview of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires...resource for planning an NEBuilder HiFi DNA Assembly mix reaction is the NEBuilder Assembly Tool which is...BioBrick® Assembly, Golden Gate Assembly, and NEBuilder® HiFi DNA Assembly. Gibson Assembly allows the...enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New England Biolabs...Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-fidelity...PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include:  Generation of...Generating constructs with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed...

...selected for by manipulating the nebulization time and pressure. Nebulization units, which can be purchased...physical fragmentation strategy is preferable. Nebulization involves forcing DNA through a small opening...

...! #1millionplasmids On to the next million! @CarolineBME @AllostEricSite @LiannaSwanson etc. Missed you...

...University of Massachusetts, Worcester  University of Nebraska Medical Center University of Pennsylvania  University...

...iTOP (induced transduction by osmocytosis and propanebetaine) to describe this method of protein transduction...