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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 21 - 40 of 76 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen...bath, and store at -80° C. Once thawed, the AAV aliquot should be used quickly. 3. Patience Is Bitter, ...

  2. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ... and how Nextflow helped us manage our data. To quote Jason Niehaus, Director of Data Science and head...

  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... containing thousands of pre-cloned gRNAs. DNA aliquots of the GeCKO v2 pooled libraries targeting both...

  5. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...without producing off-target mutations," Sha was quoted as saying when the news first broke. "Considering...

  9. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...service By Jason Nasse We regularly add new viral aliquots from our plasmid collection to provide ready-to-use...

  11. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/thaw cycles...

  12. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...tag! GFP was originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ... share your science with others.    My favorite quote about science ever comes from an interview in which...

  19. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...method you choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm...
Showing: 21 - 40 of 76 results