We narrowed to 183 results for: rato cre

...bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully...zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods in cell biology...two revolutionary large-scale random mutagenesis screens produced ~1,500 zebrafish mutants that are still...deactivation tools for zebrafish Random mutagenesis screens provided a major toehold into understanding how...et al., 2019). Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA...one-eyed pinhead mutant from the random mutagenesis screens. Microscopy-based lineage tracing can help illuminate...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...

...(Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the endogenous MECP2 gene...excision of the Stop cassette using tamoxifen-inducible Cre not only halted the neurological damage but completely...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging their combined...

... pXPR_070 Violet-excited GFP (vex) Lineage-Cre; Rosa26-Cas9 S-CHIME Knocks out pairs of genes...of genes in immune cells typically involves the creation of germline knockout mice, which is time-consuming...cells can be implanted in irradiated recipients to create mice with gene knockouts in their immune systems...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...2019). A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nature Communications...

... short hairpin) and CRUSH (Conditional RUSH) use Cre-mediated recombination to turn on or off the expression...a dual-color RNAi “sensor” mouse strain in which Cre expression causes the induction of both DsRed fluorescence...Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and... recombination events at the genetic level. Upon Cre/loxP recombination, each transgene expresses one ...Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems...SUMO tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids... all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow...

...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators... in-vivo imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral... Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here! ...

...challenge when we started was creating a LEGO stage that resembles a real laboratory. It only took one night...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at... the connection between LEGOs and science more concrete to help further unlock the potential contained...use our imaginations to mold it all into a full laboratory. It is priceless to see our “toys” gaining life...working as scientists in a custom made, colorful laboratory. When we started, the hardest part of the process...building each scenario, I go “WOW, now I have my own laboratory” (the main character is much more than a piece...

...Cas9 should be aware of the potential hazards of creating “gene drives” capable of spreading through wild...carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread through the...mistake would necessarily extend far beyond the laboratory and seriously damage public trust in scientists...does making transgenic organisms with Cas9 risk creating a gene drive? The simplest way to make a gene ...appropriate flanking homology into a germline cell can create an RNA-guided gene drive. In principle, this event...Consequently, the best way to avoid accidentally creating a gene drive is also the easiest: don't use a ... can spread through populations of transgenic laboratory organisms but not wild organisms. For example...

...determine the percent of recombination present in Cre-dependent vectors and a script that can confirm the...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...

...But CRISPRing typical mouse models, including creating Cre-dependent conditional alleles, has remained ...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems...easier to create knockout alleles in a variety of species, including the standard laboratory mouse. It...plasmid pmScarlet_C1! After creating the T7-DNA template, you'd use ivTRT to create the ssDNA construct as ...repair template is used. The original design for creating a conditional allele, or floxed mouse, from Yang... and inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-...product as a template for reverse transcription to create your ssDNA. Note that the T7 promoter will not ...

...afraid to make something concrete happen. You may have more willing collaborators than you realize. Being...careers. External collaborations are becoming increasingly important as more complex approaches to scientific...seminar to connect the imaging community and to create a platform for discussions and potential collaborations...postdoctoral work there are loads of opportunities to create communities, platforms, and to be a part of projects...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ...

...assembly decreases as the cloning complexity increases. The efficiency of recombination is increased with ... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Plasmid cloning is an essential...with successful recombination reported in all laboratory E. coli strains tested so far. The use of this...low cost and can be immediately adopted by any laboratory. All procedures are performed using the same... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures... Watson and Javier García-Nafría from the MRC Laboratory of Molecular Biology. Jake Watson is a postdoctoral...postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology. He is currently investigating...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate...Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...these projects could also lead to novel yeast creations that may not be pursued in an academic or industry...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...about new scientific techniques and cool plasmids. Creating graphics were usually the last thing I thought...professional-grade design software, like Adobe Illustrator, and I didn’t want to pay for these programs ..., I found some free design software that let me create graphics that better communicated the science in...reasons why this was the first program I used to create graphics for the Addgene blog. However, it has ... Vectr is like a pared down version of Adobe Illustrator. There’s both a web and a desktop version of ...Inkscape Inkscape is the most similar to Adobe Illustrator out of the three options for drawing your own...

...and the moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping...lists of forums on particular topics. Volunteer moderators set the rules for these forums and try to keep...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...dialogue). Luckily r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory...days). Redditors ask serious questions What the moderators did not delete or suppress were the large number...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

...the promoter (RNA polymerase binding site) and operators (response elements). Promoters are about 100 to...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with low basal activity and several tetracycline operators. Transcription can be turned on or off depending...expression Promoter from T7 bacteriophage plus lac operators Negligible basal expression when not induced. ...RNA polymerase, which is also controlled by lac operator. Can be induced by IPTG. Commonly found in pET...pET vectors. Very tightly regulated by the lac operators. Good for modulating gene expression through varied...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

...expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...light or noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider... also to minimize the damage of tissue due to increased pressure of the added liquid. The volume should...editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be observed after...

...in HEK293T cells. Laboratory tools Numerous plasmids for the production of laboratory enzymes have also...the transfer of a plasmid between two academic laboratories. While Addgene is well known for facilitating..., we shipped our first plasmid to an industry laboratory, and today we have almost ten thousand unique...Filters → Available to Industry.” Figure 1: Screenshot of Addgene's search functionality. Fluorescent... of which were developed by the James Trimmer Laboratory. Through our partnership with the Institute for...

...up with poor droplet quality. Using a droplet generator, the sample and the droplet generation oil are...are moved through small channels to create a water-in-oil emulsion containing approximately 20,000 droplets...the number of positive droplets as the dilutions increase. The ddPCR software uses the ratio of positive...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...transferring your droplets from the droplet generator to the PCR plate. Maintaining a high droplet count is important...sample. Even if you are using an automated droplet generator, you should avoid pipetting too quickly when making...parameters, here are a few things you can try: Increase the number of cycles. After additional rounds ...

...Provost’s CURE increased students’ confidence in their research capabilities and laboratory and critical...in a year-long biochemistry laboratory : Part II – research-based laboratory – a semester-long research...projects have also been proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (... Brownell, 2014; Duboue et al., 2022), as they increase student interest in scientific career paths while... Provost believes these networking experiences increase student enthusiasm, and research backs up that...research-based learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...undergraduate research experiences are a “CURE” that increases engagement by students and teachers. FEMS Microbiology...

... cancer are yielding promising results in the laboratory, and Addgene is accelerating this research by...proto-oncogenes and tumor suppressors to the accelerator and brake pedal of a vehicle, respectively. In... (now an oncogene), this is analogous to the accelerator of a vehicle being stuck down. Similarly, a mutation...as 30% of all cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations...within that pathway that are of interest to their laboratory. A single mouse click on a gene of interest will...he or she can use to study it (as seen in the screenshot below). Currently, the page includes interactive... Tackling Cancers' Drug Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used...