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We narrowed to 24 results for: sequence data Fluorescent Protein Guide

Showing: 21 - 24 of 24 results

  1. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...step: protein degradation, incomplete lysing/insoluble proteins, and protein aggregation. Protein degradation...isolating proteins from a specific compartment. If your proteins are nuclear- or DNA-binding proteins, you ...control protein (if available) and note in analysis Plasmid-based proteins: plasmid sequence not accurate...two types of gradients. Protein gradients use dilutions of your sample or protein of interest to understand...  Figure 1: A protein gradient. The density of the bands, which increase as protein concentration increases...in order to release the proteins from their binding. If you’re seeing protein aggregation, you may want...you need very accurate protein measurements, consider using an unstained protein ladder, which will run...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... from Addgene’s sequencing reactions (‘Addgene Sequences’).  These sequences are provided in FASTA format... your research! Sequence information: Depositors may provide full or partial sequence information for ...be made available as ‘Depositor Sequences’. Additional partial sequence information is also available from... such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find...most of these pages, we've written this post to guide you through them and make the best use of all the...auto-generate a plasmid map based off of the plasmid sequence and feature information given by our depositors...example) and can be analyzed using Addgene’s Analyze Sequence tool. Excellent, how can I check if the plasmid...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...unreliability: cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...technology, it is important to show through qualitative data that they are a long-term solution to reproducibility...atlases/literature and is unrelated to the target protein, we categorize the antibody as having non-specific...atlas will enable scientists to have an intuitive guide for checking their staining and neuroanatomy without...you are interested to get involved by submitting data, sending antibodies, and/or working with Dr. Wu ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....

  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...well as the type of transgene delivered: fluorescent proteins, optogenetic sensors, CRISPR elements, etc...BBB via interactions with a similar protein called LY6C1. This protein is expressed in both BALB/cJ mice...psst… don't forget to submit your validation data to our Data Hub once you’re finished!) Good luck and happy...exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic... it, cross the BBB through an endothelial cell protein called LY6A (Hordeaux et al., 2019; Huang et al...
Showing: 21 - 24 of 24 results