Skip to main content
Addgene
Showing: 21 - 40 of 510 results
  1. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... if there is statistical significance among data sets. Significance is a numerical value which quantifies... about outliers or data spread within those data sets. Measures of dispersion – standard deviation/.... 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test assumes your data...parametric tests. Parametric tests are suitable for data sets that “play by the rules” and which we have certain...example, if an ANOVA analysis on a five-sample data set yields statistical significance, you won’t know if...comparisons are performed. For example, if your alpha is set at .05, then 5% of the time the statistical test ...
  2. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...meetings is to give honest, constructive feedback, set tangible research and career goals, and celebrate...techniques to finally figuring out a tough experiment, sets a positive and encouraging tone for the meeting....overwhelmed or confused about what’s coming next. Goal setting and career planning help alleviate the anxiety ...suitable careers based on your current interests and skillset. With this information in hand, you can then formulate...
  3. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...suggestions into a final version of the document. Set and manage expectations When the researchers involved...Therefore, it’s crucial that everyone meets early on to set expectations for the output that will come from the...other accountable to these expectations once they’re set by checking in about them at your recurring meetings...
  4. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...easy to pipette samples in and out of it, you can set up a procedure for it using the Biomek FxP. For example... example, we added a cooling block to our set up. The cooling block is used to keep our cells from warming...transformations in the same amount of time by hand! Setting up a basic protocol on the Biomek FxP is pretty...the shortest amount of time. Racks of 48 vials are set up on the bed of the Liquid Handler and we program...
  5. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local pub at...yourself. Pick a topic that is close to your heart, set up a blog using a site like WordPress and start writing...involve the public in your research projects, you can set up a citizen science project. Answering the big science...Museum has downloadable resources to enable you to set up your own citizen science project. Science communication...
  6. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...took some playing around to figure out all the settings and tool options, but I found Vectr to be fairly...Biorender watermark, which is usually ok for informal settings like lab meeting presentations. You can download...entire internet for scientific graphics. The tools settings let you tailor your search to particular types...
  7. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...LEGO laboratory sets so we had to purchase the big LEGO classic box, the Big Bang Theory set, and then use...potential is endless One night, while opening a LEGO set with my husband, I thought about how the limitless...
  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...cytometry to analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely...cell or on its surface. Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC,...cytometer's software will allow you to adjust this setting as needed. To figure out the appropriate voltages...default, a sample on the flow cytometer (before you set the first gate) will be represented through FSC-A...-A vs. SSC-A, so most people use that setting. Figure 2: As the cell passes through the laser beam...
  9. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...biological sciences—whereas industry postdocs have a set time limit. At AstraZeneca, this is three years. ...easier to make that transition from an academic setting—especially if job applications require teaching...There are “difficult personalities” in every work setting and no manager will ever be perfect, but the chances...good manager do tend to be higher in an industry setting. Scientific community and mentorship: As an industry...
  10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...Differentiating male and female flies is essential for setting up genetic crosses. At the end tip of their abdomens... hatching from their pupal case (~9 days after setting up the genetic cross). You can tell when pupae ...Animals are diploid except for their germ cells (sets of genomic material are contributed by mother and...meiosis. This can sometimes be a handy feature when setting up genetic crosses. More importantly, this knowledge...
  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...relative to a 'normal' sample with known copy number set as the expression baseline. Copy number is then determined...sample relative to your normal control - you can set up your calculations so that your assay output number...numbers should be as small as reasonably possible and set using the variance seen within multiple runs of the...CAR15.) Each droplet contains a primer and probe set for both the gene of interest and the reference sequence...
  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...since primers are insert dependent   PCR set-up Setting up colony PCR reactions is nearly identical...primers to detect the presence of your insert; 2) set up a standard PCR reaction (primers, dNTPs, polymerase...antibiotic for selection.   Lysing bacteria and setting up PCR reactions: The remaining bacteria-water ...know there could be something wrong with the PCR setup and/or the primer design. Use a negative control...
  13. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...right corner of PubMed to navigate to your account settings. Next, click on the link in the “NCBI Site Preferences...badges you can add and use your new PubMed account to set-up emails to alert you when papers are published ...
  14. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...help the research community. Set expectations for your productivity Set a routine and stick to it. Plan...to your kids' needs. Plan ahead. Think about and set up activities that kids can do more independently...
  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...to switch off a few minutes. The host could also set aside Breakout Rooms to divide the audience into ...sparking new ideas. Social no-science-y meetings Set up a brief bi-weekly meeting where all lab members...collaborations remotely outside of the lab meeting setting. Check out the blog post “Fostering Communication...
  16. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...safe. None of this is conducive to a classroom setting, especially for a school with a limited budget....provide them to their students. My desire to create a set of low-cost, easy-to-use educational biology activities...could be widely adapted and used in a variety of settings. If you are a biology teacher at the elementary...
  17. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...business-focused?" The reason is that I knew that the mind-set of the employer was not such that he/she would be...they sought out opportunities to expand their skill sets?", “Have they succeeded in what they have done?”...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...
  18. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...fit well with your goals. These first hires will set the tone and atmosphere that future hires will look...the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for some quick advice and resources...Connie Cepko was surprised by the variety of skill sets she needed to acquire as a professor. “Being a PI...
  19. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...using pulses of light. By activating a neuron or set of neurons, you can see how it contributes to behavior...are many examples where you want to activate two sets of neurons and see how different parts of the brain...we can use light to more specifically activate a set of neurons, maybe we could treat patients suffering...this idea been around? Boyden: Optogenetics is a toolset that lets you turn the electrical activity of neurons...
Showing: 21 - 40 of 510 results