Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 74 results for: sgrna

Showing: 21 - 40 of 74 results

  1. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...Once supplied with a guide RNA — whether it’s an sgRNA or a crRNA:tracrRNA duplex — Cas9’s two lobes move...yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   The REC lobe binds tightly to ...yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   DNA sequence recognition Once ...yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue; target DNA strand, light gray; non-target... and marks the transition back to a DNA duplex. sgRNA, dark blue; target DNA strand, light gray; non-target...yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue; target DNA strand, light gray; non-target...

  2. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...assemble multiple unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This ...for the multiplexing of sgRNAs against unique genomic targets or multiple sgRNAs against the same target...target to ensure efficient cutting. Beyond sgRNAs, MoClo can also be used to test a variety of Cas enzymes...

  3. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus ...cumbersome in vitro synthesis of single guide RNAs (sgRNAs), are time-consuming, have been typically optimised... the in vitro-transcribed single guide RNA (IVT sgRNA) approach (Dewari et al. 2018 and Figure 2). Not...construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure 2: Cas9 RNP complexed with 2...

  4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...or HDR. EGFP validation of sgRNAs: Can evaluate component delivery and sgRNA efficacy by cloning in genome...you will need to deliver. Minimally, one or more sgRNAs and Cas9 are required for any application. If you...

  5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...around 20 base pairs long. Using a single guide RNA (sgRNA) to create a double-strand break works well because...locus (Ho et al. 2012). For large-scale screening, sgRNA libraries are useful for targeting thousands of ...uses a nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90% in human cell...limited (likely due to its long, negatively charged sgRNA), RNP delivery was much better with Cas12, which...Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...including the cell line/tissue type, enzyme system, sgRNA library and other details relevant to screen result...gene-level scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...does just this by including an additional sgRNA. Using this sgRNA, the prime editor nicks the unedited strand...increases indel formation slightly. Designing the sgRNA with a spacer that only binds the edited strand,...Structured RNAs with improved stability Traditional sgRNAs are protected from cellular degradation by Cas9...efficiency ✓ ✓ ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand Favor ...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...research with a simple question: how do Cas9 and sgRNA interact with and dissociate from a DNA target? ..., requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson is a...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system, Cas9 is...known as the guide RNA (gRNA) or single guide RNA (sgRNA). Throughout the remainder of this post, we’ll refer...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and cloning sites for inserting the guide...pyogenes CRISPR chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance from...

  16. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  18. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...trace files to the TIDE tool online along with your sgRNA sequence. The TIDE software will generate a graph...along with the estimated editing frequency of your sgRNA-Cas9 combo. Pro tip: When designing your PCR, ensure...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... the Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8... a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they...
Showing: 21 - 40 of 74 results