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We narrowed to 43 results for: stem loop

Showing: 21 - 40 of 43 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...new multiplexed CRISPRi and CRISPRa system for human pluripotent stem cells By Jennifer Tsang CRISPR gene...To overcome these variations in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute..., stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! New from the...improve utility of the system for use with low affinity variants of TEV. The new system has been demonstrated...cells Nanobody purification CRISPRi and CRISPRa system for hPSCs The CRISPR corner New from the viral ...studies.  Find the plasmids to set up your screening systems at Addgene! Schematic of the evolution platform...piggyBac plasmids include a mRFP reporter. With this system, they could quantify and track cells expressing...

  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...guide RNAs with internal barcodes (iBARs) in their loop region. After infecting cells with sgRNAiBAR library...of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell types and organisms...

  4. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...tracking progress, and starting a writing group. Loop Habit Tracker app for Android  This app lets you...that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero is also another free citation ...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...that aptamer insertion in the gRNA tetraloop (a four base hairpin loop motif within the gRNA) resulted in...School developed a dCas9-based multicolor labeling system to visualize chromosomal loci in human cells (Ma...sites on different chromosomes in living cells, the system requires high-copy chromosome-specific loci in ...stability. First, they began with the Broccoli aptamer system to visualize the gRNA in living cells. Broccoli...inserted an octet of MS2 hairpins into the gRNA tetraloop and went through subsequent design iterations ...measure inter-locus distances. They tested this system out on chromosome 19 by targeting 46 repetitive...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...serotypes that vary in their RBE and T-shaped hairpin loop sequences (Wilmott et al. 2019). Most ITR sequences...Viral Vectors 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's...rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https:/...

  7. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional transcription factors to...for vemurafenib resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator) uses...well as more difficult conditions such as model systems and primary cells. References 1. Doench, John ...Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification in gene expression and...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...with the Cas13a molecule through a uracil-rich stem loop and facilitates target binding and cleavage through...Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA and crRNA to facilitate...high specificity (Gootenberg et al., 2017). This system, dubbed SHERLOCK (depicted in figure 1) has been...specific PFS, making it a very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing...characterization of diverse class 2 CRISPR-Cas systems." Molecular cell 60.3 (2015): 385-397. PubMed PMID...

  9. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...simpler in structure than Cas9’s; only a short stem-loop structure in the direct repeat region is necessary...and tested? Class 2 CRISPR systems, including the type II Cas9-based system, require a single-component...Cas9 Type II CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest ...RNA-guided endonuclease of a class 2 CRISPR-Cas system. Figure from Zetsche et al., 2015. How was ... the multi-subunit complex employed by class 1 systems. A putative new class 2 nuclease, Cpf1 (CRISPR ...several genomes and is classified as a type V CRISPR system. Like Cas9, Cpf1 contains a RuvC-like endonuclease...this information, they turned to a cell culture system to see if any Cpf1 nucleases would exhibit in vivo...

  10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...photo-sensitive LOV2 domain from A. sativa into a loop region of the Acr protein AcrIIA4. In the dark, ...(Acr) proteins potently inhibit type II CRISPR systems including the popular S. pyogenes Cas9 (Rauch et...light-sensitive protein domains, they have developed a system to control CRISPR-Cas9 activity using blue light...took the light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach...interaction studies easier with a 2in1 vector cloning system Article contributed by Michelle Cronin  Listen ...Grefen lab introduced the “2in1” vector cloning system - a Gateway compatible approach to simultaneously...be cloned into an entry vector. The 2in1 cloning system contains two entry vectors (pUC57-L1L4 and pUC57...

  11. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed...cell-based assays and mouse models. The dTAG system The dTAG system pairs highly selective small molecule FKBP12F36V...contains the full description of the dTAG system. The dTAG system was employed to evaluate degradation of...which we refer to as the degradation tag (dTAG) system, to co-opt the endogenous ubiquitin proteasome ...protein. We are particularly excited about the dTAG system as we can use it to evaluate the effects of rapid...traditional genetic approaches. In addition, the dTAG system is versatile and can be used in both cell-based...novartis.com). Considerations when getting the dTAG system up and running in your lab Confirm the functionality...

  12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...to the CIB1 (cryptochrome-interacting basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains...soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise mapping... integrated into a Ca2+ -modulating optogenetic system (OptoSTIM1), caused two-fold faster changes in ...

  13. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...target various RNA molecules - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer, and more - to ...Baby Spinach core embedded in a single, extended stem-loop, contiguous with the sgRNA core (see image above...activation is capable of promoting induced-pluripotent stem cells (iPSCs) to take on a “neuron-like” phenotype...organelles, a cell cycle indicator for mouse embryonic stem cells and a series of inducible promoter constructs...CRISPR-Cas9 system to create a tool that is quick, versatile, and robust. Dubbed the LACE system for light-activated...via the CRISPR system. The ease and flexibility of the LACE technology makes this system widely accessible...gene expression by creating the SunTag system. The SunTag system, named after the "stellar explosion SUperNova...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...protein that specifically detects a specific RNA stem loop-structure (Querido and Chartrand, 2008). Finally...naturally occurring co-factor binding proteins, systems wherein a protein binds a synthetic, fluorescent...strategies works best for a specific biological system under anaerobic conditions needs to be examined...covalently label the peptide with a fluorophore. Both systems are a hybrid between a genetically encoded tag ...protein labelling in vitro and in vivo." Molecular BioSystems 12.6 (2016): 1731-1745. PubMed PMID: 26960991...in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed PMID: 24360272...

  16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...Julius Brennecke. They developed a method that uses loop-mediated isothermal amplification after reverse ...expression plasmids available for many expression systems: bacterial, yeast, and mammalian. These plasmids...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...iPSC generation Deriving iPSCs (induced pluripotent stem cells) through the expression of transcription factors...this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting...reprogram human fibroblasts into induced pluripotent stem cells. By replacing wild type SOX2 with either a...extra-chromosomal telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted. Reduction...nickases, and gene activation systems as well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression... I CRISPR systems, but only recently have inhibitors for the popular type II CRISPR systems, which include...Gateway-compatiable toolbox for studying the nervous system The Cole lab recently deposited the Tol2 Gateway-Compatible...

  19. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ... is expressed as inverted repeats separated by a loop region that facilitates the formation of a hairpin...‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with an Internal Marker... Morgens DW, Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for... Goshima G (2012) An Inducible RNA Interference System in Physcomitrella patens Reveals a Dominant Role...

  20. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...engineering in 2012.  Many of these advances have stemmed from the goal of reducing off-target Cas9 activity...Phillips-Cremins JE (2019) LADL: light-activated dynamic looping for endogenous gene expression control. Nat Methods...their CRISPR system. This common optogenetic technique can be employed in other split systems where light...HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave a target...activation using dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light ...Gersbach’s lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...proteins. The goal of both groups was to create a system that would use light to turn on and off gene expression...
Showing: 21 - 40 of 43 results