...procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow.   ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)...

...of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ...

... synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce...many suitable dyes to choose from and you can opt to do-it-yourself with a kit or purchase it commercially...but you can still choose what dye to use  What to do once you’ve chosen a gRNA and modification(s) Congratulations...

...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used...

...technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise...

...visited Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena...

...unlikely you’ll be able to do them all for every western blot assay you run. How do you know which steps to...visualize them with a conjugated antibody. If you do not need such precise measurements, you can use a... double ladder on the lefthand side. Whatever you do, be consistent and document it.   Did it work? Save...directly from tissue or supernatant, and you’re doing the purification and cross-adsorption in lab, consider...adjusting your exposure time first, since you can do that without having to re-run the western.  Remember...the data and what level of accuracy you’ll need to do that confidently.  Once you’ve run your western, ...

...Additional Resources AAV Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for...

... Reddit AMA Here are some concrete things you can do to develop those transferable skills while you are...relies on a division of labor Serve on a committee and do something big like plan a conference Recruit many...

...csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when planning...Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides...knock-in efficiency (<2% positive by ICC for the tag) or do not see any cells positive for tag insertion, you...et al. 2018), however, if you have any questions, do not hesitate to contact me at [email protected]...