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We narrowed to 772 results for: INA

Showing: 191 - 200 of 772 results

  1. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...developments.  Further, this post may contain technical inaccuracies or typographical errors.  No action should be...patents and letters of recommendation. EB-1A extraordinary ability category The EB-1A - is similar to national...journal editor) of the work of those in your field. Original contributions to the field that have had a major...include the fact that you are a classically trained ballerina who has performed at Lincoln Center. Your case...of permanent, tenured or indefinite (no fixed termination date) employment at a university or institution... the same or allied academic field Evidence of original scientific or scholarly research contributions...management, alliance management, business development, finance, and operations. To learn more, visit www.propelcareers.com...

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...alterations. These studies utilize a catalytically inactive form of Cas9 known as dCas9 (Jinek et al., 2012... mammalian cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting...this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic gRNA, is incubated...DUX4, a transcriptional regulator that when inappropriately expressed can cause facioscapulohumeral muscular...non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing." PLoS One 10.4 ...chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...sure that the sample is pure and free of any contaminants that could affect the measurements. You'll need... if not, since buffer components can affect the final readout. When preparing a standard curve, change...even when the same protocol is being followed.  Finally, standard curves must be validated for each run...software to calculate the data’s coefficient of determination, R2. R2 is the square of the correlation between... to increase the accuracy of your experiment.  Finally, carefully analyze the data and use the trendline...Addgene's Ready-to-Use Antibody collection Addgene's Recombinant Antibody Plasmids collection  ...

  4. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...was over 300 years ago. Teaching by example No examination of the state of science would be complete without...story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was not included amongst...spends some time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene...undermining of the scientific process and the marginalization of science, particularly public health and ... observations and suggestions are not so much inaccurate as already somewhat dated. Zimmer could not have...continue to be a “critical consequence of ...the marginalization of science,” we know now in the summer of 2020...

  5. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...a wash solution removes contaminants while nucleic acids stick around. Finally, purified total nucleic...sample can be treated with RNase to remove RNA contamination and you can use a phenol chloroform extraction...extraction to remove remaining proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended...into this mixture to absorb the nucleic acids. Containinants bind cellulose less tightly than nucleic acids...with 1M phosphoric acid and re-used, but trace contamination could interfere with interpreting the results...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... digital PCR is a sensitive assay, so cross contamination into a no template control (NTC) well is common...reduce aerosols that could potentially lead to contamination. Optimize your PCR For a good starting place...place on what PCR parameters to use, see Lock’s seminal paper on AAV titration (Lock et al., 2014). If you...tool for titration and integrity evaluation of recombinant adeno-associated viral vectors." Human Gene Therapy...PMID: 29548643. Lock, Martin, et al. "Absolute determination of single-stranded and self-complementary adeno-associated... PubMed Central PMCID: PMC5915492. Witte, Anna Kristina, et al. "A systematic investigation of parameters...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous...transduction of both the CNS and PNS.That is until the Gradinaru Lab at Caltech stepped up to the challenge. Find...is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this technique...short DNA sequences that contain all possible combinations into a region of the cap gene. In this special... one RGB AAV and red, green, or blue are the predominate colors expressed (figure 4c).   AAV Vector...therapy treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways...

  8. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ... input and polling, Blugene's new virus friend finally has a name! We thank you for your participation...* Aavery! Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene...names and we’ve recently chosen four names as finalists. Now, we’re asking you to help us make the decision...meaningful names…. Watch the video to find out the final four! (Note: For cases where the same name was submitted...submitted by more than one person, we selected the finalist based on the first time of submission.)     ...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...cytosine or adenosine deaminases to facilitate a single base pair edit. (Cytosine deaminases edit C-to-T and...and adenosine deaminases edit A-to-G.) In short, the impaired Cas9 localizes the deaminase to where the...This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik.  ...choose this system.  Fig. 1: Cytidine deamination takes place on the free strand of DNA and converts...

  10. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... of these CRISPR systems. The original pCas/pTargetF system Our original pCas/pTargetF system consists...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers... to yield pEcCas. In pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ...of the pEcCas/pEcgRNA system Compared with the original system, the pEcCas/pEcgRNA is superior in several.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters...
Showing: 191 - 200 of 772 results