We narrowed to 821 results for: LAS

...curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you use a plasmid from ...variety of plasmids in the repository. And last, but certainly not least, we often feature new plasmid technologies...process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation ...comparing two plasmids, we recommend reviewing the cited papers to determine whether the plasmid has been ...collection or if you think a particular plasmid or type of plasmid is missing from our collections pages...spotlight some of the plasmids that have come through our doors in our “Hot Plasmids” section. We’ve also... added the Plasmid of the Day feature to our home page - each day we showcase a new plasmid that you might...

...within the plasmid backbone to generate R-mAbs of different subclasses, including subclasses that were ...important collection.  Creating the Plasmids To begin constructing the plasmids for expression of mAbs, the Trimmer...expression plasmids were DNA sequenced to determine the VH and VL sequence. These plasmids have been deposited...scientific community with the plasmid sequences available from each plasmid webpage. Importantly, the methods...different subclasses, but there are also secondary antibodies that are specific to each subclass. Approximately...mouse mAbs are IgG1 subclass (Manning et al. 2012), which limits the use of subclass specific secondary...original hybridoma. By using subclass specific secondary antibodies and subclassed switched R-mAbs, multiple...

...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...without plasmids at Addgene To find out whether scientific articles associated with Addgene plasmid deposits...those containing plasmids that the authors deposited with Addgene and those without plasmids deposited with...2,596 of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations ...with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...Papers without Addgene Plasmids in Journal (E) Percent of Papers with Addgene Plasmids in Journal (F) Weight...more interestingly, could we tell if depositing a plasmid with Addgene causes a paper to get cited more? ...

... Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology ... indicates that a plasmid has been requested 20+ times   A red flame means a plasmid has been requested...answering plasmid requests can be a time-consuming chore for labs that have popular plasmids but lack ...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...scientists around the world choose to share their plasmids through our repository since we know biological...

...this case, plasmid copy number is the input and gene expression is the output. The input, plasmid copy number...to decouple gene expression and plasmid number. In a suite of plasmids modified from pSC101 with copy ...of all types of plasmids, the stabilized promoters were also tested in different plasmid backbones that...stationary phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, ...RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid by altering...genome or a plasmid. Thus, a stabilized promoter can allow a system to be moved from a plasmid to the genome...special collections page Download the Plasmid 101 eBook See all plasmids deposited by the Voigt lab ...

... beginning. In most classrooms, the laboratory experience is focused on classical embryology techniques...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments... cloned into plasmids to investigate for each of these planarian species. These plasmids all have a PR-T4...insert the cDNA into the desired plasmid backbone. The Sánchez lab plasmids allow students and new labs to...feedback etc. please write to cuttingclass@stowers.org and if you make new plasmids for investigating different...Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids for other developmental models...of specific gene expression. The 49 deposited plasmids enable students to study gene expression and functions...

...determine the ideal excitation). Lasers can be purchased through Laserglow, which caters to optogenetics ...users and adjusts the lasers appropriately (tuned for optogenetics). These lasers are relatively cheap ... run you about $2-3,000/laser. However, an important caveat is that one laser can stimulate only a single...apparatus and thus individual laser stimulation is essential. In terms of the laser stimulation itself, the ...control output of several lasers simultaneously, can be used to set the laser output without manual manipulation...patch cable to connect to the laser and rotate 360° without a loss of laser power. More recently, wirelessly-controlled...neuroscience validation experiments we discussed last week are only half of the battle when using optogentics...

... Can I use my favorite plasmid with this protocol? Maybe. Not all plasmids will work for the one-pot...strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will be degraded...those in the plasmid. Again, if they are in the opposite direction, both strands of the plasmid will be nicked...map of your plasmid. If the sites are in opposite directions, you just need to use a plasmid with BbvCI...Mutagenesis to Mutate Your Plasmids Resources on Addgene.org Find Plasmids from the Whitehead Lab Use.... This technique relies on the generation of a plasmid library that expresses all desired variants of ... a selective pressure winnows the pool down to plasmids expressing variants with optimal function. High-throughput...

...lentiviral plasmids Addgene's most popular retroviral plasmids Addgene's collection of AAV plasmids Addgene...split across several plasmids (often three), for safety purposes. The primary plasmid with the delivered...often a gene) is called the transfer plasmid while the other plasmids with packaging or replication components...of adenovirus plasmids Resources on the Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral...For safety and production reasons, multiple plasmids with many components are used to package lab grown...can be intimidating at first to look at so many plasmid maps and protocols! In reality, there are many ...components are called helper plasmids. Below, we’ll breakdown the vector components first by virus and then...

...deposited plasmids. To resolve QC issues, assembly issues, and difficult-to-sequence plasmids, other sequencing...it to the plasmid information or depositor-provided sequences to confirm the correct plasmid components...Addgene The Power Behind NGS Plasmid Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional...general overview of the QC process at Addgene. All plasmids go through this same initial QC process using ...impractical for whole-genome (and often, whole-plasmid) sequencing.  Prior to short-read sequencing, Addgene...information on this process, see our blog post on NGS plasmid quality control. NGS is generally cheaper if you...it typically results in high accuracy for full plasmids. However, NGS might not be the best choice for...