We narrowed to 884 results for: ica

...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-...general (1). Science and politics? Science is an apolitical process for seeking knowledge. The process begins... again. No part of this process is inherently political so why is there a march in Washington, D.C., the...States government? Politics and science are intrinsically linked: governments exist in reality and science...as evidence of climate change, the safety of genetically modified organisms (GMOs), the absence of a link... grant in the last five years as polled in the American Association for the Advancement of Science) believe... it.  3) Linking scientists to one particular political party while alienating the other While scientists...

...conflict is not always what was communicated but how it was communicated. The next step is to come up with...of lapsed communication. Making some assumptions about how something should be communicated is necessary...set of transferable skills, coupled with your technical skills, can open new career opportunities for ...conflict can also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully...scientist. And some amount of conflict is actually critical in the business world. Companies and organizations...traced back to one of a few trouble spots:  Communication (or lack thereof): Information sharing gets ...organization gets, and creating new avenues of communication can create as many problems as it can solve....

...well as protease sites to facilitate protein purification. We hope this kit will allow you to quickly ...for a variety of synthetic biology or protein purification studies in E. coli. 800 and 1900 Series If you...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...: Čelešnik, Helena, et al. "Biosafety of biotechnologically important microalgae: intrinsic suicide switch... Rogers, Jameson K., and George M. Church. "Genetically encoded sensors enable real-time observation ...and Charles E. Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics... Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31-37. pCri system The Gomis-Ruth...

...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...choosing antibodies   Complexity is a key factor Biological systems tend to be complex with a variety of ...

...show all publications by an author, total number of citations, and the number of publications that heavily... staying on top of the ever expanding body of biomedical literature! PubMed PubMed is the classic search...read the abstract or full text of the paper, publication date, a relevance score, and type of article....that term. You can also set up alerts for new publications from a particular author. Saving papers Papers...interest.  Best uses Accessing the vast body of biomedical research papers. Twitter Scientists often use... work, so it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 ...organizations, and universities often tweet about publications in the fields of research they support. Monitoring...

...et. al.). Physical versus functional titer Viral titers usually come in two flavors, physical or functional...the target cell’s genome. Physical titer The two most common assays for physical titer are direct p24 measurements...functional. Physical titers measure the amount of viral particles in a sample and are usually based on the...produced can actually infect cells. These assays typically involve infecting a target cell line with your...particle. Consequently, titers based off of p24 quantification tend to be overestimates since they can include...quantify the amount of PCR product that comes from amplification of the original plasmid which can over-inflate...methods tend to be more accurate than measuring physical titer, there is a risk that they may underestimate...

... its nuclease activity, catalytically dead Cas9 (dCas9) has many applications, the most well-known being...characterizing the 4D nucleome. Future modifications and applications Ma et al. developed this technique ...plasmids from this publication at Addgene. A protein-tagging system for signal amplification in gene expression...technique published every week! One of the newest applications is a colorful system that uses fluorescently... The technique successfully resolved loci with physical map distances of 75 and 2 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart... et al. anticipate that the technique will be applicable to studies of cell cycle progression, epigenetics...

... offers a significant improvement and simplification of the original technique, specifically in terms ...interaction with limited biological materials. Problems with Chip-seq ChIP-seq typically requires a large amount...material for a ChIP-seq experiment can be a major technical hurdle.   ChIP-seq requires  cross-linking during...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...Washington. CUT&RUN doesn’t require fixation and significantly decreases background noise, reducing the read...development using the Drosophila wing as a model. A typical ChIP-Seq experiment requires around 1,000 wings...dissections. With CUT&RUN, we have had success with significantly fewer wings, such that enough tissue for an ...

...improving expression of nutritionally and therapeutically significant proteins. In this project she aims to...pests. By developing biological agents such as insecticidal proteins, the use of chemical pesticides may be...scientists work on projects spanning from the identification of plant compounds and insecticidal proteins...get tested for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of... yeast capable of desaturating fatty acids. Identification of Insecticidal Proteins Linh Bao Ton (M. Sc...one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is pQE-30-HyPer3 (2) which...

...techniques in the biological sciences and constitute foundational elements of biomedical research. Traditional...Restriction Enzymes + - PCR Amplification + + Digestion + + Gel Purification + - Ligation + - Transformation...Molecular cloning is the isolation, insertion and amplification of a recombinant DNA without sequence alteration... multi-step process consisting of digestion, purification, ligation, and transformation. While restriction...sequences for base pair overhangs, insert and vector purification, and, most importantly, excessive time requirements...is ligation independent, it does not require purification of insert or vector products, nor does it require...Digestion products are then transformed directly into chemically competent E. coli and transformants selected ...