We narrowed to 802 results for: LAT

... should talk to if ruminating the trials and tribulations of the modern PhD program. Don’t be fooled! ...Medical School. He studies developmental gene regulation with Dr. Angela DePace in the Department of Systems...

... out in time - from assembling the boxes, to inoculating tubes, and to packing and shipping. Every single... This massive scale reagent sharing led us to calculate how much time we have saved researchers. In a ...

...the order they appear in the plasmid and their relative position to the insert. Example, if you have a... immediately followed by its position (number) relative to the start Methionine (Met) followed by the ...

...Join a newsletter to stay up-to-date on all the latest news and encourage your peers to do the same. ...reagents stable for years. In fact, some scientists speculate the -80 °C setting might have been a marketing...

...attracting and keeping diverse talent in science related careers. Failure to strengthen the talent pipeline...science career resources Browse all blog posts related to science careers Download the Science Career ...

... genes in clonal populations and to knock-out thousands of genes in mixed population of cells as part ...

...and was originally focused on becoming an MD but later got obsessed with research. Chapter 3: How all basic... basic research ends up being translational. Ex: the discovery of GFP. Chapter 4: Goals of the Center ...

...to develop a plan for moving forward. Sooner or later enough people will be vaccinated and we’ll have ...spend time caring for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening...

...These tips were written early on in the pandemic. Later in the year, Joanne Kamens followed up with a blog...mapping, DNA/protein databases, DNA sequence manipulation, and primer design. Best wishes for the new...

...future of science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter...Schofield, C., … Laflamme, C. (2024). A consensus platform for antibody characterization. Nature Protocols...

...Associate Director of the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic...Associate Director of the Genetic Perturbation Platform at the Broad. His mom always told him that he ...

...A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor...

...mentors and mentees have fulfilling, productive relationships. I will be sharing what I’ve learned along the...many had examples of very productive mentoring relationships with advisors and others who helped shape their...

...Neuromodulators like dopamine and norepinephrine have important functions in the brain but have been ...generating a measurable signal after electrical stimulation with a single pulse, while nLightR gave a measurable...

...gives a presentation on what they do and how it relates to other teams at the company. This provides new... I know some fear that remote work would feel isolating, but that has not been the case for me at Addgene...

...Offices at universities to make COVID-19 research-related plasmids available more broadly: to scientists ...resources on the Addgene blog Browse blog posts related to COVID-19 Learn more about the Ginkgo COVID-19...

...function, and C. elegans overexpression plasmids to upregulate their expression in specific cells. With a more...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...

...citing plasmids from Addgene help others find them later if they need to use them in their own work, it also...should make it easier for them to find your work later. We also hope that this feature will decrease redundancy...

...distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously established...

...is pictured here…she started from a blank white slate and developed this over 30 minutes, which was fun...this type of experimentation to the eventual translation to the clinic and cures. There is no doubt that...

...game for anyone looking to quickly and easily manipulate specific genes. But what if you want to study...said they were able to produce a very diverse population of cells within a lab dish in a matter of a few...

...bacteria, yeast, mammalian, etc.). To individually isolate a protein, there must be a special feature about...S-Transferase (GST) is a 26 kDa tag which can be isolated with glutathione resin. Like MBP, it can be removed...

... data, and I was given a fantastic spreadsheet template to build out a report on. I was able to understand...cultivates trust between coworkers and that trust then translated into this incredible experience.  Of course, ...

...may be most effective. Once drug candidates are isolated, they can be validated for specificity to their...before time and money gets put into them in those later stages. Target identification Not all diseases ...

...selectable marker. Each insertion line can then be isolated and screened. Depending on the marker, traps can...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...

...Improvements In a world where funding cycles shift, regulations change, and supply chains strain, Addgene remains...breakthroughs. None of this progress happens in isolation. It’s made possible by a shared commitment to ...

... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...

...endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

...inactivation, protein degradation, and/or the use of chelating agents that can be introduced in the purification...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

...person, and helps improve your interpersonal relationships with people you’re collaborating with remotely...external collaborations Browse our science career related blog posts ...