...will help with the next steps.   References and resources References Al-Allaf FA, Tolmachov OE, Zambetti...Choose a restriction enzyme that will give you different band patterns for the recombined vector backbone...

... if I would have formulated specific comment differently if I would be speaking to a colleague in the ...of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the...own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, ...

...why this protein was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a...

...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (...2013). However, the brightness of Brainbow was inherently low. This is because the stochastic and combinatorial...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

... together as useful and productive, forming a different constellation of interests and skills than what...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...biology. Beyond that, she is also a fan of baseball, French horn, and rhubarb. Follow her on Twitter @Pam_Hines...

...sections and brought in more focus on the list of conferences I presented at and placed them under headlines...before I went for the interview. So reading for my current sales position interview was just as important....mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

...lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for the third base of the PAM so that it now ...and Intensely Desensitizing ChRs) differ from currently used ChRs in that they cause a rapid and almost...researchers identified MerMAIDS from their analysis of different publicly available metagenomic data including ...

...incorrectly known as “three-parent babies,” where fused genetic material from intended parents is “rehoused” in ...self-police” or whether the media and academic conference response to He created a positive feedback loop...

... improve tuberculosis care and control. In her current research, Sheen aims to identify new genes related...related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic...microfabrication technologies to generate a self renewable sensor, a bacterium that can sense contaminants...

...academic community by funding their travel to a conference of interest. In this post, we’re proud to announce...for human milk oligosaccharides Fatima Enam is currently a graduate student in Thomas J. Mansell’s lab ...cost-effective way to measure the production of HMOs. Current HPLC-MS and fluorophore-based technologies are ...

...new and useful information, and invigoration - a renewed energy for continuing the project after leaving...a sense of the employee’s workload, updates on current projects, and offer feedback. The employee should...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

... to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...

...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...

...interest. They also “showed that different guide RNAs have different abilities to turn genes on and off..., transformations require electroporation. References & Further Reading: For more information about...

...data to prove it is worthless. The OSER assay is currently the best way to test a fluorescent protein’s propensity...papers should be used with caution, since the difference between studies is substantial (e.g, the poor...conclude, we recommend that you try a couple of different fluorescent proteins when protein tagging is the...

...Rounding out the remainder, 2% of postdocs who weren’t going to get a salary increase prior to the injunction...that institutions have sent, with the lack of transparency and poor communication of their plans (many ...guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. ...

...has lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address...proteins that interact with an RNA of interest. Current labeling methods are not ideal for in vivo work...The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, D.S., Neela...

...sources. Mode of communication Different modes of communication take up different amounts of mental bandwidth... you're asking of your audience and when things aren't working out, adjust the ask.  I used to do a lot...

...recent positions outside of the lab. Granted, I currently work on the Scientific Support Team here at Addgene...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...how you approach new challenges.     Know your strengths! Research experience presents ample opportunities...

...Association for Creatine Deficiencies (ACD) is a parent-let non-profit founded with the mission to support...newly independent faculty member studying sex differences in thoracic aortic disease,” Prakash says. In..., the lab hopes to capture the signalosome of different receptors upon stimulation. “To study cellular...

... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...et al 2016 and Wong et al 2015. Alan Wong is currently an Assistant Professor at the School of Biomedical...systems. He can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy...

...plasmid) and any other important features that differentiate this plasmid from its predecessor. Important...sequencing provider. Each sequencing service will have different requirements for volume and concentration, and...Resources on the Addgene Website View our Plasmid Reference Pages Get Plasmid Backbone Information from Our...

...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...constructs, assemble standard plasmids parts in different combinations, or other new and exciting applications...constructs in a single, high-efficiency step. References: Sequential amplification of cloned DNA as tandem...

...creates strong, well-directed meaning and delivers strength to the sentence. Below are the typical sections...an opportunity to summarize the limitations of current state of the art technologies. Therefore, take ...address some, if not all, of the limitations of current methodologies. This section sets the stage for ...