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... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

...treatment (Stephens et al., 2019). If sufficient gene transfer and knock-in is achieved, producer cells could...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...

...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...

...Red-shifts the wavelength (yellow derivatives) A206K Interferes with dimer interface (monomeric derivatives) ...earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...

...on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes identified...Knockout Library are available from Addgene! References 1. Morgens, David W., et al. "Genome-scale measurement...

...existing comprehensive mutagenesis methods, PFunkel offers the best combination of library coverage, mutational...mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis...

...similar properties to Broccoli despite binding to a different small molecule. Notably, like Broccoli, Red Broccoli...reports on the next additions to aptamer soup! References Alam, Khalid K., et al. "A fluorescent split ...

...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...more at his webpage or follow him on Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman...

...are, their role in the immune response, and the different antibody isotypes found in mammals.      Subscribe...

...guide. Print out the guide and use it for quick reference when you're designing your next virus experiment...

...support use of their antibody tools. Addgene also offers a growing collection of educational resources on...

...viral vectors and their elements; resources on different types of vectors, capsids, and tools; and helpful...

... Jennifer Tsang...

...introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources on the Addgene...

...101 eBook is designed to be a helpful desktop reference. Each chapter brings together all of our 101 blog...

... Jennifer Tsang...

...facilities management to website development, tech transfer, lab work, and more. It's this diverse group of...

...priority given to paid content regardless of user preference. Addgene is a values-driven nonprofit. Recent...

...CRISPR, Addgene gave to me: CRISPR activation and interference screens for primary human T cells🎵 On the 3rd...

...post on lunch. Why should our new blog be any different? If you still need an extra push, there’s evidence...

...biology protocols Check out our molecular biology reference pages ...

... Jennifer Tsang...

...Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly...

...David Baker, Demis Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani...

...Addgene. Learn How to Analyze a Restriction Digest References 1. Henrici, R. C., Pecen, T. J., Johnston, J....

...Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

...because it enables lentiviruses to infect many different types of cells. This means researchers who use...

... constructs, why not let us know? Fill out our referral form and we’ll take care of the rest. Know of...

...New Protocol Videos View Our Molecular Biology Reference Pages ...

...engineer and producer for the Addgene Podcast.     References 1.Tang, Jonathan C.Y., et al. “A nanobody-based...

...YouTube channel and watch it now! (Bonus: Tim kindly offered to finishing answering all submitted questions ...

...is a great step up, but I needed a way to show different aspects of the data simultaneously. That’s why...

...Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA Chapter 4 - Developing...

... Jennifer Tsang...