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Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

Mycoplasma contamination? Find out where it comes from, how to detect it, prevent it, and remove it from your tissue culture experiments.

Oct. 8, 2020, 1:15 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

March 21, 2017, 2:30 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Addgenie Emma Markham describes her winding career path and provides advice on how you can find a great job after undergrad.

Jan. 12, 2016, 3:30 p.m.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

March 21, 2024, 1:15 p.m.

Recent developments using CRISPR/Cas9 editing with C. elegans, focusing on the use of an embryonic lethal selection marker to select for knock-in.

Dec. 17, 2020, 1:30 p.m.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

March 14, 2017, 2:30 p.m.

Learn about key considerations when conducting optogenetics experiments in mice, including how to pick and delivery an opsin and stereotaxic surgery tips.

Sept. 10, 2015, 2:30 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.