Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 787 results for: lat

Showing: 381 - 400 of 787 results

  1. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence...neuron more positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

  2. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...let you know before these releases happen (and congratulate you on your paper!). And as always, we won’t.... But this way, as soon as we become aware the related journal article has been published we’ll get the...the plasmids’ citation when the paper comes out later. Or, if a project isn’t working out as planned, ...citation to the final journal article version any time later. We recognize that a preprint isn’t necessarily ...

  3. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...imaging to show where the bacteria localize in relation to internal organs. The limit of detection for...

  4. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...to detach from the constant stream of thoughts related to lab research and gain fresh perspectives. Such...encourages exploration of tangents or seemingly unrelated ideas. It's beneficial to carry a notebook at ... identify areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives...everything is a miracle.” This quote encourages us to contemplate our approach to life and how our viewpoints influence...

  5. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling and structure-guided...which had a total of 31 amino acid replacements relative to CFP484. Because the excitation and emission...combination results in a 2.6-fold increase in signal relative to mCerulean imaged with a standard CFP set. mTFP1...fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID...

  6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...multiple, small colonies After streaking LB agar plates, you may notice that there are both large and small... bacterial cultures with lentiviral vectors, the flat white colonies contained the full plasmid, whereas...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https...

  7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    Published
    June 22, 2021, 1:15 p.m.
    ... encode spikes found on all of the different circulating variants. This allowed us to create pseudoviruses... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera... shocked [with these results] because we were simulating the live virus experiment,” says Balazs. “But...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

  8. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several... neuroscience was carried over and allowed to percolate within a new intellectual environment. Over time...perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental ... health policy implications arising from sport-related concussion.   More resources on the Addgene blog...

  9. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...faster with technology transfer offices (TTOs). The latest of these efforts is the Golden Stopwatch Award ...Team! Why MTAs Matter Though they may seem like boilerplate drudgery to some scientists, MTAs are important—they...

  10. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ..., increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...Root System Architecture of Arabidopsis HapMap population in Amsterdam. Currently, she is a postdoc at ...expression patterns in plants with enhanced sodium accumulation in their roots and (3) development of tools ...

  11. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...Careers has developed a web-based, career-planning platform called the Individual Development Plan (IDP) and...using multidisciplinary, collaborative, and translational approaches for scientific tool and technique... of the project. For example, how can future regulations impact your work? What design variables need ...Patent process Business Models Communication Regulatory knowledge Networking Blogging Writing...

  12. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...thought there was room for improvement and a year later it became quite clear that others thought the same... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community...newer tools may still prove somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target...tools allow the user to save the results and return later or share them. Community exclusivity: Different...sites, scores and the statistics that go into calculating them. For this, some online tools such as Cas-OFFinder...

  13. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...changes of non-target genes and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

  14. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius, ...specific loci based on the guide RNA sequence. A year later, they developed CRISPRainbow (Ma et al., 2016), ...chromosome-specific loci in the genome and the guides are relatively unstable.   Figure 1: CRISPRainbow labeling... proteins. (B) CRISPR-Sirius used to visualize relative locations of the subtelomeric region of the pericentromeric...

  15. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022...therapeutic antibody itself or as impurities in the formulation. Efforts to predict T cell epitopes focus on ..., and can be used as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes...

  16. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...these small DNA strands can form concatemers in later steps. This new NEB kit includes a step that both...the DNA and prevents it from forming concatemers later. The "FS" stands for Fragmentation System and this... an NEB drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the...understand? How does the information I’ll be learning relate to me as an individual and Addgene as a company...

  17. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...tried to find an app that could help me with calculations. This is how I came across the Gibco Cell Culture...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... was the molecule of interest (analyte), and accumulation of ATP activated GFP expression (receptor), ..., then the transducer could be a microscope, plate reader, or any other instrument which measured GFP ...advantages over other mechanisms. For one, many viral platforms, such as AAV or retrovirus, can either permanently...Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in...

  19. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...of your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...expression control Researchers are always wanting to manipulate gene expression – turn it up, turn it down, knock...PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the gene therapy landscape. Sig Transduct...

  20. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ... in the nucleus. The Roth lab showed that this platform accurately identified 120 known GPCR targets demonstrating..., the receptor fused to APEX2 catalyzes the biotinylation of proteins near the GPCR. These proteins are... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine...
Showing: 381 - 400 of 787 results