Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 772 results for: INA

Showing: 201 - 210 of 772 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...packages navigated customs and arrived at their destination in a timely manner and at the correct temperature...have partnered with six distributors (Table 1), in China, Korea, Japan, Brazil, India, and Mexico. Table ... Country Served Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 ... e-nnovation Life Sciences 2020 India Sinapse Biotecnologia 2022 Brazil   In some cases...agreement with a distributor for that country. In China, direct shipments weren’t able to clear import control...materials and accept deposits from scientists in China. They were also able to help with sharing information...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...growing amount of interdisciplinary research while at the same time increasing interdisciplinary citations in...continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads to everyday... solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies...interactive chart where you can see the yearly interdisciplinarity index of research for single or multiple ...2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research by the numbers." Nature 525.7569 ...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ... and decide whether you will create an N- or C-terminal fusion based on your experimental needs. Equally...have reported that FP cysteine residues form inappropriate disulfide bonds in the secretory pathway (Figure...localization. For example, the formation of inappropriate inter-chain disulfide bonds between FPs blocks...and blue variants that can be used in various combinations. For example, with standard fluorescent microscope...can label multiple proteins of interest with a combination of green and blue or cyan and yellow moxFPs. ...postdoctoral fellow at the University of North Carolina at Chapel Hill. Under the co-mentorship Blossom...

  4. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...  The Addgenies will choose up to four finalists, and the final winner will be determined by popular vote...cloistered consideration, and with much debate, we have finally narrowed it down to three choices.   Fig...unveil the winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, ...Additionally, anyone whose submission is included in the final four will receive some rockin’ newly designed Addgene...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...-open. In the two closed states, integrins are inactive, with low affinity for ligands. When integrins...toolset IPI’s anti-integrin toolset comprises 26 recombinant antibodies (rAbs) either produced de novo using...into two groups. Seven are reconstructions of the original hybridomas as new reproducible rat or mouse rAbs.... Based on the established applications of the original hybridoma monoclonals, we anticipate that our ...other RGD-binding integrins, individually and in combination, showing that among the three αV integrins expressed...an expanding collection of readily accessible recombinant antibodies and plasmids. To encourage collaboration...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...Cas12a activity gets activated. This unleashes indiscriminate ssDNA cleavage activity which cleaves the supplied...extracted from a sample and subject to isothermal recombinase polymerase amplification (RPA) using a commercially...amplification is done at one constant temperature, eliminating the need for thermocyclers. This is followed ...contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ reporters that exhibit fluorescence...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...protrusions and overlaps (arrows) after blue light illumination. Scale bar 25 µm. Images adapted from Mahlandt... 10, 30, 50, and 100 times the activity of the original, generated using directed evolution (Tei et al... can hydrolyze phosphatidylcholine (PC), the predominant membrane lipid, to phosphatidic acid (PA) or ...GFP-PASS (green). After 30 minutes of blue light illumination, substantial PA accumulates in the plasma membranes...other enzymes. Conclusion We hope this post has illuminated some of the many tools available for controlling..., & Hehnly, H. (2023). Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Nobody EVER complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts... at [email protected]." You can find his original blog post below! --Ed.  Eight years ago, I decided...proper consideration. 3. Present an easy to follow seminar See item 2. Attendees will include grad students.... This isn't the time to go into detail. Finally, state how successfully completing the research...applying for It's not about you and how you will finally be granted the faculty position you have craved...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher...strand invasion, a key step in the homologous recombination pathway. The Waldman lab at the Georgetown University...for the generation of knockins via homologous recombination with a homologous repair template. It is this...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele... mechanisms and consequences of cohesin gene inactivation in human cancer.     References 1. Russell, ...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...1977, Sanger sequencing, also known as chain-terminating or dideoxy sequencing, was a major breakthrough... of all possible lengths because extension is terminated upon the incorporation of a ddNTP. Researchers...gels are classic techniques that allowed for many seminal scientific discoveries (5, 8). While newer techniques...techniques. For example, restriction mapping led to recombinant DNA technologies that are now commonplace in ... Alan R. Coulson. "DNA sequencing with chain-terminating inhibitors." Proceedings of the National Academy...al. "Large-scale and automated DNA sequence determination." Science 254.5028 (1991): 59-67. PubMed PMID...
Showing: 201 - 210 of 772 results