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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 320 results for: POR C

Showing: 201 - 210 of 320 results

  1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...reaction takes an hour or less to complete at 50 °C. After an hour or so, the sample is immediately ready...PMID: 25861928. 3. Jiang W, Zhao X, Gabrieli T, Lou C, Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome...primers for PCR amplification of fragments to incorporate such regions of homology. This video gives a ...

  2. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...ask at least one question to clarify the situation; C = confirm that you have understood the speaker accurately...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, ... of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...Genetically modified organisms like transgenic mice are important tools for understanding the function of cell types...enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The ...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ..., G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and... Mikkers, J. Kool, L. van der Weyden, A. H. Lund, C. H. Wilson, R. Rance, J. Jonkers, M. van Lohuizen,...useful for genetic engineering approaches. Most importantly for this technology, however, is that the transposase...should be tested in the cell line of interest. Importantly, by varying these ratios, you can either maximize...obstacle, a positive selection cassette can be incorporated, and used during the differentiation process...homologous recombination, which results in the incorporation of your specific edit into the host genome. ...transposon sequences.  The use of PBx is particularly important to avoid any possible random re-integration from...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies...10.1080/19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view from...Reproducibility First, let’s review why validation is so important. Antibodies are a critical component of the researcher...or plan your own validation experiments, it’s important to know what you are aiming to achieve. In general...as whole cell lysate or tissue sections. It is important to confirm that your antibody is not binding to...proteins bound to the target protein, so it’s important to understand if protein:protein interactions ...

  6. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...Plans Aren't Just More Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon...and PI, it’s easy to miss out on beneficial opportunities or to find oneself unsure of the next steps....planning meetings with each trainee to create a supportive, growth-oriented lab culture. The aim of these...the overall tone of the meeting is almost more important. As DePace says, science is full of “terrible ...postdoc training. On the other side, the PI has an opportunity to check in with lab members, determine how to...

  7. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...PubMed Central PMCID: PMC1559948. Brusca, Richard C., and Matthew R. Gilligan. "Tongue replacement in ... please! At Addgene, we love finding ways to incorporate science themes into our fun activities and Halloween...body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...

  8. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ...Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer of BCL11A subject to...work was with TALENs, in collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

  9. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Addgene.   References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A systematic identification and analysis...physical scientists are underrepresented. It’s important to note that these results could be biased for...domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized ...

  10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...attach the gene of interest to either the N-or the C-terminus. Even if a researcher’s gene of interest ...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...
Showing: 201 - 210 of 320 results