...born before 1985, you might remember going to the store and buying CDs when you wanted to hear a piece of...technology makes it easy for scientists to share, remix, store, and track these essential components of the scientific...useful in perpetuity.  Second, plasmids are easy to store and share, making recombinant antibodies the most...

...plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand breaks...very long ncRNAs is impractical. Instead, tags or stop elements can be inserted to disrupt the transcription... adjacent genes are complex and not yet fully understood, so it is important to carefully select the target...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...but labeling ceases when these two bait proteins stop interacting. This feature is particularly useful...

... In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication programs... and money to a full-time graduate course, UWE Bristol offers an intensive 4-day Masterclass in science...or upload your data to Zooniverse. The Natural History Museum has downloadable resources to enable you...

...so many scientists who were potential Addgene requestors and depositors. China is in one of the top 10...good job of seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab ...can view public circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq...

...Women’s Hospital/Harvard Medical School and then Boston University (institutions where I’ve worked as a...became interim Chair automatically as it was an understood expectation of any Vice-Chair. Following an official...Juliet Moncaster is an Assistant Professor at the Boston University School of Medicine and is the former...

...methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and...(2005): 443. PubMed PMID: 15908923. Johnson, Christopher M., and Alan D. Grossman. "Integrative and conjugative...

... inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated mostly from abnormal and...PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of stem cell research. Best Pract...Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med, 2004. 350(13): p. 1353-6. PubMed...

...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer... comments below! Special thanks for Dr. Dan Stone and Harshana de Silva Feelixge at Fred Hutchinson...

...spectral flow and can be thought of as a series of histograms flipped onto its side and compressed into a heat...for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody binding...: Secondary AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's...