We narrowed to 884 results for: ica

...Attempts to remove excess bone surgically cause the body to “heal” the surgical site with even more bone that...therapeutic biologic design (ADCs) along with pre-clinical analytical development. His areas of expertise include...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...to get in shape. After three kids, I had put on typical middle age weight and “exercise” consisted of taking...named AJ Gonzales who suffers from Fibrodysplasia ossificans progressiva (AKA FOP). This terrible disease ... But, there is HOPE. FOP is a genetic disease typically caused by a single R206H point mutation on the...growth in mouse models. As this biologic moves into clinical trials, there is new HOPE for AJ. But, as we in...

...the gut side of things, the authors optigenetically and chemically excited a class of enteroendocrine ...behavior, or the animals being monitored were physically hooked up to invasive, cumbersome systems during...and stimulate or manipulate responses through neurological means like electric stimulation, drug administration...programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers across multiple...fibers. As important as its capabilities are the practical considerations of actually using it - the device...to mCherry to the brain tissue. They performed optical stimulation using NeuroStack and the fibers as ...as previously described, then measured electrophysiological potential afterwards. This measurement trended...

... This allows them to replicate viral plasmids with the SV40 origin of replication. To generate a virus...proteins at high levels. In a case where clinical applications of recombinant proteins are necessary, HEKs...serum-free growth, which significantly reduces the cost of production. Research applications There are two areas...isolating the cell line all the way to modern applications. What the HEK is it?   HEK cells were first...chromosome is present in this line, making it genetically female. HEKs are generally cultured as an adherent...cell biology, there are thankfully many other applications that they are useful for! What the HEK is it... cellular research tools have been optimized specifically for and in HEKs. These systems built around ...

...Scientific Publication, where diverse approaches to peer review were discussed. Science communication opportunities...developmentally programmed regulation of DNA replication and gene transcription during vertebrate embryogenesis...with people, nurturing networks, as much as with technical knowledge. I leverage a breadth of knowledge to...I am constantly teaching – often explaining a technical piece of research to others outside that specialty...and jobs. At Science, which is published by the American Association for the Advancement of Science (AAAS...punctuation can be wildly funny as well as financially critical. Those who work on the publishing side of science...There is a rich and active community of science communication professionals who are thinking about, for example...

... also be perturbed by supraphysiological expression levels. BiFC quantification This is where BiFC outshines...Miller-Rhodes from the University of Rochester Medical Center.  You’ve probably heard of Forster Resonance...PIPs, resulting in a fluorescent readout.   Applications for bimolecular fluorescence complementation...2018), the generation of such resources in less genetically tractable organisms lags behind. BiFC can also...resulting from the assayed PPI. This high-throughput application is much more straightforward to carry out because...protein construction, cellular expression, and quantification (Kerppola, 2006, 2013). These are described...to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...

...BglBricks: A flexible standard for biological part assembly. Journal of biological engineering. 2010;4(1):1. Pubmed... diverse functions, and thus, is used in many biological fields. In synthetic biology for example, high-throughput...elements to generate non-natural tools such as novel biological circuits or sensors. Given the expanding palette...develop diverse fluorescent reporters is a useful application of high-throughput cloning. MXS Chaining is one...plasmids for fluorescence imaging or flow cytometry applications in mammalian cells. The modules used in this...transgene expression, or a variety of other clever applications. In one example, modular constructs generated...methods are suited for particular downstream applications. The tradeoffs between various cloning methods...

...biology. Therefore, my lab is dedicated to development of new biological technologies. The locus-specific...been working on development of technologies for biochemical analysis of genome functions such as transcription...technology, which uses locus-tagging and affinity purification, in 2009. However, iChIP requires insertion ... genomic regions can be easily isolated for identification of associated proteins, RNAs, and other genomic...describe this technology and some of its potential applications? Fujii: We generated a series of retroviral ...researchers to use enChIP in a wide variety of biological systems. We are currently developing several ...interference-PCR (ORNi-PCR) method to suppress PCR amplification of specific DNA sequences. Thus, we've made ...

...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...neuroscience. In fact, precise light-based control of biological processes is highly useful in other fields, including...consistent, and controllable pulses of light. Complicated patterns of light intensity and wavelength can... also be used to engineer new pathways with sophisticated gene expression controls. Most cell types do...system to develop a bacterial camera that prints a chemical image, as well as a genetic method for the computational...light; these two systems can be coexpressed for sophisticated control of gene expression. An E. coli light-sensitive...; only two plasmids are required per system. Applications of these systems The large dynamic range of ...

...fields where communication and writing are key skills. Other fields, often highly technical or skills-based...proactively address questions about your application. These could be logistical, like briefly explaining a gap ...excellent communication skills” try giving an example, like “I have extensive communication experience...usually yes! A good cover letter won’t hurt your application, but it can certainly help you stand out.  In...consider cover letters an important part of the application. Even within an organization, there can be variance...good cover letter!) As with any part of the job application process, it's a good idea to talk to people in...work on [phenomenon X], I read several of your publications on [specific topic]. I am extremely interested...

...antibody interaction and/or the downstream application. Typically, if the target protein will be detected...a protein’s stability or post-translational modifications, or study how a target protein interacts with...Immunoprecipitation Overview An immunoprecipitation reaction is typically carried out in one of two ways. Both methods utilize.... 2) An antibody immobilized on a bead binds specifically to the blue triangle protein but not the other...Protein A and Protein G are not suitable for all applications, such as protein isolation from serum. In this... or magnetic beads with commercially available chemical agents. Directly conjugating the primary antibody...interactions To prepare for an IP, samples are typically lysed in a non-denaturing buffer containing non-ionic...