We narrowed to 833 results for: Cat

1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   Blog Post

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   Aug. 6, 2024, 1:15 p.m.

   ... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...

2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   July 6, 2017, 1:59 p.m.

   ...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow...

3. Antibodies 101: Buffers, Storage, and Conjugates

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   Feb. 1, 2022, 2:15 p.m.

   ...compensation.    Working with antibodies can get a bit complicated, but hopefully this helps you successfully select...

4. New and Upcoming Viral Vectors - May 2020

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   Blog Post

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   May 28, 2020, 1:15 p.m.

   ...availability date or to be notified when a particular catalog item is available for order please email [email protected]...

5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

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   Dec. 6, 2018, 1:34 p.m.

   ...interactions of proteins within these structures. Enzyme-catalyzed proximity labeling (PL) uses a promiscuous labeling...

6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   Sept. 15, 2020, 1:15 p.m.

   ... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

7. Plasmids 101: Inducible Promoters

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   Jan. 18, 2018, 2:34 p.m.

   ...prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein...

8. Plasmids 101: Screens vs. Selections

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   Nov. 10, 2022, 2:15 p.m.

   ...In fact, selections are often inappropriately categorized as screens! Why the confusion? The commonality...

9. CRISPR 101: Non-Homologous End Joining

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   Blog Post

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   April 16, 2015, 3:45 p.m.

   ...Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous...

10. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

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    Feb. 18, 2014, 4:22 p.m.

    ...This is a chance to practice leadership and communication skills. The leader also provides materials such...

11. Antibodies 101: Fab Fragments

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    Blog Post

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    Sept. 5, 2024, 1:15 p.m.

    ...fragments described above can be used for similar applications and have similar advantages, though with some...

12. Transferable Skills Guide: Public Speaking

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    Blog Post

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    April 20, 2023, 4 p.m.

    ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...

13. Plasmids 101: The protein expression toolbox

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    Blog Post

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    Feb. 16, 2023, 7:05 p.m.

    ...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor...

14. dTAG - You're it!

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    Blog Post

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    June 21, 2018, 2:06 p.m.

    ... and we will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG...

15. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

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    Sept. 24, 2019, 12:55 p.m.

    ...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

16. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

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    Aug. 1, 2024, 1:15 p.m.

    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

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    Oct. 6, 2020, 1:15 p.m.

    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

18. Antibodies 101: Flow Compensation

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    May 16, 2024, 1:15 p.m.

    ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

19. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

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    Oct. 12, 2017, 2:26 p.m.

    ...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

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    July 13, 2017, 2:30 p.m.

    ... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

21. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

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    Feb. 7, 2019, 2:16 p.m.

    ...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

22. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post

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    Dec. 1, 2016, 3:30 p.m.

    ...in line 4 of Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed...

23. Plasmids 101: Multicistronic Vectors

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    Blog Post

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    Sept. 9, 2014, 8:20 p.m.

    ...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and...

24. Tips for arabidopsis transformation

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    Blog Post

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    Oct. 25, 2018, 1:23 p.m.

    ...Agrobacterium tDNA insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds...

25. Negotiating Work and Life: How to Find the Joy

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    Blog Post

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    March 20, 2014, 3:04 p.m.

    ...other activities. Take care of yourself. Take a vacation alone with your partner – no kids. Get a massage...

26. Antibodies 101: The Four ELISAs and When to Use Them

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    Blog Post

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    July 25, 2024, 1:15 p.m.

    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are...

27. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

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    Feb. 18, 2020, 2:15 p.m.

    ... written up in mSphere. A post-meeting survey indicated that the program was largely a success, but also...

28. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

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    July 23, 2019, 12:46 p.m.

    ...Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely...

29. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

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    March 12, 2019, 1:37 p.m.

    ...anticipated outcome, and payoff for each aim. Learn to communicate your story with only the most salient details...

30. Supporting Reproducibility with a Connected ELN

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    Blog Post

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    Jan. 17, 2019, 1:38 p.m.

    ... both accept deposit of data associated with publications into one of five general purpose open source...

31. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

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    Sept. 5, 2017, 1:58 p.m.

    ...many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...

32. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Feb. 22, 2017, 3:30 p.m.

    ...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...

33. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post

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    July 12, 2016, 2:30 p.m.

    ...interested in using CRISPR to develop therapeutic applications that better human health.     References Cong...