In Vivo Biotinylation of Bacterial Fusion Proteins
Type
Blog Post
Published
Jan. 25, 2018, 2:09 p.m.
...of an experimental protein-ligand interaction approaches that of GST-glutathione.
Biotin-based immobilization...lack of site selectivity typically diminishes the apparent affinity for the ligand, especially if the modified...located near the ligand binding site. A selective approach entails the use of the enzyme biotin ligase, which...