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...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...are particularly interested in facilitating collaborative science, and making it accessible to all people...

...Download Addgene’s Science Career guide to learn more about career paths and things you can do to prepare. ...can adapt to your kids' needs. Plan ahead. Think about and set up activities that kids can do more independently...leading up to your defense less stressful. Think about reproducibility best practices Support your future...

...scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our Plasmids 101 eBook! ...to produce sgRNA for CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify...Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology...

...enable future discoveries. Read on to learn more about Xin and Fatima's work and keep checking back in ...the 2017 Winter qBio Meeting. You can learn more about Xin’s work with photoswitchable components of the...Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent Proteins Use Light...

...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

...aren’t reflected in the common components outlined above for gamma-retroviruses. While not required for lentiviral...and helper plasmids for all the viruses discussed above (gamma-retrovirus, lentivirus, adenovirus, and adeno-associated.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ...

...20 amino acids. But in your quest to learn more about your protein of interest, you may find the available...system-wide effects. And on top of that you need to worry about getting high enough expression of your protein of...on their website specifically so people can talk about GCE. In fact, their first protocol is available ...

...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

...chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime have... inhibit neurons. You can find information more about chemogenetics and a concise table of DREADDS and...Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics Resources...

...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

...“I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently less...efficacy as well as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are ...Some of the RNA stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications...

...blog Learn about the advantages and disadvantages of using monoclonal antibodies Read about other techniques... subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101: What is an...

...off target effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA...have been several systems to do this but most are labor-intensive, require multiple fluorescent proteins... Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with...

...19 blog posts Learn about other COVID-19 plasmids available at Addgene Read about SARS-CoV-2 detection...the full suite of time consuming, costly, and laborious QC for all several thousand plasmids at once. ...

...blog Learn about other CRISPR methods for bacterial genome engineering   Learn more about lambda red ... genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications...

... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...genetic cargo. Typically, when researchers speak about AAVs, they are actually referring to rAAVs. ITRs...Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org ...

...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...Hébert's lab. Additional Resources Find out more about the Rare Genomics Institute and this year's BeHeard...