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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Abo;

Showing: 401 - 420 of 836 results

  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our Plasmids 101 eBook! ...to produce sgRNA for CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify...Additional Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology...

  2. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...enable future discoveries. Read on to learn more about Xin and Fatima's work and keep checking back in ...the 2017 Winter qBio Meeting. You can learn more about Xin’s work with photoswitchable components of the...Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent Proteins Use Light...

  3. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

  4. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...aren’t reflected in the common components outlined above for gamma-retroviruses. While not required for lentiviral...and helper plasmids for all the viruses discussed above (gamma-retrovirus, lentivirus, adenovirus, and adeno-associated.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...20 amino acids. But in your quest to learn more about your protein of interest, you may find the available...system-wide effects. And on top of that you need to worry about getting high enough expression of your protein of...on their website specifically so people can talk about GCE. In fact, their first protocol is available ...

  6. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...off target effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA...have been several systems to do this but most are labor-intensive, require multiple fluorescent proteins... Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with...

  9. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

  10. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...blog Learn about the advantages and disadvantages of using monoclonal antibodies Read about other techniques... subclasses: IgA1 and IgA2.  You can learn more about isotype classes in our Antibodies 101: What is an...

  11. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime have... inhibit neurons. You can find information more about chemogenetics and a concise table of DREADDS and...Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics Resources...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...blog Learn about other CRISPR methods for bacterial genome engineering   Learn more about lambda red ... genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications...

  14. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...genetic cargo. Typically, when researchers speak about AAVs, they are actually referring to rAAVs. ITRs...Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org ...

  16. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

  17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not require a tracrRNA...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ... the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development and ...

  18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...Hébert's lab. Additional Resources Find out more about the Rare Genomics Institute and this year's BeHeard...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

  20. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...Narrowing the field After working your way through the above steps, you may find that you still have a number...advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical support? An...validation is equal and you should think critically about what data is and is not shown on an antibody website...
Showing: 401 - 420 of 836 results