Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 847 results for: CAL;

Showing: 401 - 420 of 847 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...present in the cell, it can also introduce non-physiological RNA-protein interactions due to sample processing...its rate of biotinylation. The improved enzyme, called BASU, is derived from a BirA* homolog found in ... interactions in events that occur on a short timescale, such as during drug action or cell signaling....t be used to study endogenous RNAs at their physiological expression levels; however, knock-in of BoxB... by 90%, suggesting that QKI is a host protein critical to Zika replication. Human genetic disease. RaPID...

  2. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...ribonucleoprotein, and AAV.  Read the paper in Nature Chemical Biology Find the AsCas12f1 plasmids   Selective... Mike Lacy The retroviral-like protein PEG10 specifically binds its own mRNA to facilitate its packaging...of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular... the prime editor, yielding PEmax, to work synergistically with epegRNAs and PE4 and PE5. These enhanced...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...and success. Customer and technical support Our customer and technical service brands continue to ... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...positive, supportive culture, we are attractive to the local employee pool and we get a lot of successful candidates...connections and even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern...

  4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ... department at the University of Massachusetts Medical School. We are working with postdocs at other institutions... and all 6,000+ postdocs at the University of California, who had a contract - negotiated by their union... December 1st. Salary inconsistencies across geographical regions When we originally started gathering...salaries. Postdocs at the Salk Institute in La Jolla California are not getting a raise. Meanwhile, in less densely...changes in science. Follow him on Twitter @BiophysicalFrog and Future of Research @FORsymp.   Additional...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...requisite for a functioning lab, but it is for a scaleable lab. When you need to track the location, quality...our second example we’ve added some simple but critical logic. Some steps fail “hard”, they reset the ...interchange them easily as needed. In this case, the physical considerations of the laboratory make object composition...science_name`. You'll need to decide given the technical details of the problem you're solving, but consistency...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT...the two strands are ligated, resulting in the reciprocal crossover event. This event can have three general... features with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of...putting the loxP sites on either side of a gene (called “floxing”, for “flanked by loxP”), will permit ...where "N" indicates which bases may vary from the canonical sequence. Among these are loxN (GtATACcT), lox2272...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...and on self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory...receptors (NRs), their coregulators, and their physiological endocrine ligands. Nuclear receptors and their...the diet. Additional members of this family are called orphan receptors because their ligands remain unknown...circadian regulation, as well as in important physiological states such as adipogenesis and macrophage activation...widely used today.  The sheer abundance of these critical research tools is a double-edged sword, however...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...past decade, primary cells remain difficult to genetically edit due to toxicity from electroporation or ...some cases) and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this...Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9...microscopy in HeLa cells expressing COX8a-mStayGold. Scale bars 10 μm (main), 1 μm (inset). Image reused from...versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this...

  9. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...the transfection method (i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of ...unconcentrated prep. For example, at Addgene we typically concentrate 100-fold meaning that a 10 cm2 dish...experiment requires a certain volume of virus, consider scaling up by either increasing the number of 10 cm2 dishes...be optimized prior to starting. We recommend calculating the ratio of the surface area of the larger vessel...lentivirus is the cell line that is used for titering. Typically researchers will choose a relatively permissive...

  10. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...expression observed with wild type CoChR, soCoChR localizes primarily to the cell body with very little signal...engineered microbes, used coffee grounds could be economically repurposed as starting material for the bioproduction... FLINC biosensors for live-cell imaging of biochemical activity Article contributed by Angela Holmes... live-cell imaging of the dynamic changes in biochemical activity within the cell. Their Fluorescence ...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET...

  11. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...methyltransferase DNMT3A allows researchers to easily add biochemical marks to genetic regions from which where they...eventually, develop treatments, we need tools to specifically edit methylation sites.  Step aside, transcriptionally...interact with transcriptional machinery to modify local gene expression, confounding any potential conclusions... DNA. When dCas9 is bound to a target CpG, it physically blocks DNMT1 from that site. So, when DNMT1 methylates...Szyf 2021.   Let me leave you with a few practical considerations: Design your gRNA to overlap the...

  12. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ... from E.coli, named TadA7.10 in ABE7.10)  a catalytically impaired Cas protein (dCas or Cas nickase)  ... the eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated ABE8s...accidental insertions and deletions (indel) by the catalytically impaired D10A nickase from ABE7.10 can be a ...the authors created ABE8 constructs using a catalytically “dead” S. pyogenes Cas9 (dC9-ABE8.x-m/d). The...even in adult patients. In order to analyze the clinical use of ABE8 for primary human cell editing, the...

  13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... staying on top of the ever expanding body of biomedical literature! PubMed PubMed is the classic search...interest.  Best uses Accessing the vast body of biomedical research papers. Twitter Scientists often use...papers, and more. Saving papers There isn’t technically a way to save papers on Twitter, but you can ... contain a short summary of the topic and a chronological list of highly cited papers that contain your...also just follow feeds curated by Meta. Feeds automatically update as papers are published, similar to Facebook...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...can contain different neurotransmitters. Using genetically encoded fluorescent proteins coupled with Fibronectin... endogenous synaptic proteins allowing them to localize to the synapse and minimize extraneous fluorescence...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet... genes whose protein products have distinct localizations. Figure 2: Split-wrmScarlet labeling ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...using either loxP or lox511 sites, leaving two identical sites on one end of the DNA fragment (Figure 1...recombination event then excises the DNA between the identical loxP or lox511 sites (Figure 1, bottom). Since...presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead of BFP.... rescue with in the antisense orientation (let’s call the gene “GeneR”). The key to this experiment is...information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A ...

  16. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...workplace, and pursue leadership opportunities at the local, institutional, regional, and national levels. These... first at Brigham and Women’s Hospital/Harvard Medical School and then Boston University (institutions...issues faced by postdocs and institutions are not local but are national issues. I applied and was successfully...other people mostly online and on monthly phone calls. This has its challenges as meeting in person is...Chair then stepped down, I became interim Chair automatically as it was an understood expectation of any Vice-Chair...

  17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...and solutions to research problems. This movement calls itself the Open Science movement and is part of ...and the reagents are too expensive to make it practical - but we have members looking at how to re-use...don’t throw old equipment away until you ask your local makerspace if they want to hack new life into it...somewhere near you. The place to start looking for your local space is the Hackerspace website. Markerspaces differ...fun with technology, so feel free to contact your local space and get involved! Derek Jacoby is a PhD ...

  18. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...different questions than those on the prep calls, but often the prep calls provide good ideas for interesting ...possibly with an organizer) should set up a 30 minute call with each panelist to get to know their style and...better at remembering these things on a relaxed phone call than when first confronted on a stage in front of...long introductions. The moderator asks questions, calling on 1-2 panelist for each question in a way that...

  19. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...BV510 and AmCyan have almost identical emission spectra but may be called BV510 on one cytometer and AmCyan...for one another. This overlap in spectra is often called "spillover." For example, if your flow cytometer...shift towards the X axis to become a perpendicular vertical line.     In general, most spillover between samples...research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources on the Addgene blog Introduction...

  20. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...the right fit for you without the financial and logistical stress that accompanies traveling for campus ...Visits After receiving applications, universities typically invite a select group of students to continue ...and each other. Before COVID-19, financial and logistical obstacles could make touring potential programs...in-person interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage...format of interview from a travel day to an online call in response to challenges on their end. Though the...
Showing: 401 - 420 of 847 results