We narrowed to 520 results for: EMB

1. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   Published

   Oct. 26, 2017, 1:59 p.m.

   ...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing...

2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ...investigating DNA repair pathways.     Additonal Corn Lab Members who worked on this publication:         References...

3. Synthetic Photobiology: Optogenetics for E. coli

   Type

   Blog Post

   Published

   Sept. 8, 2015, 2:30 p.m.

   ...other plasmid-based systems, which will enable the assembly of larger systems. The work of Schmidl et al. ...

4. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   Published

   Dec. 18, 2014, 4:06 p.m.

   ...skills, and the job you are applying for. Also remember that a resume is typically no more than 2 pages...

5. Advice for Scientists Starting a Lab

   Type

   Blog Post

   Published

   April 29, 2014, 1:43 p.m.

   ...scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ...

6. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...

7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are...

8. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification...

9. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...side to your right as you use them can help you remember what's been added and what hasn't. Developing ...

10. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...

11. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is...

12. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ...

13. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...specific locus to create a knock-out in human embryonic stem cells.  Find GEmCherry2 reporter plasmids...

14. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ..., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available...

15. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries...

16. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically...

17. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    Published

    June 29, 2017, 1:06 p.m.

    ...colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences...

18. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for...

19. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ...

20. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ... in addition to testing their performance (2). Remember that although digital PCR is a great screening...