We narrowed to 680 results for: Eng;
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TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....scientific paper, you may feel it’s overwhelming or challenging. So instead, let’s focus on practicing with a...dive into applying these four steps elsewhere, engaging in this practice, and perhaps modifying it as ...
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CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....can I use it? CasPEDIA operates like many search engines, featuring both a general search bar tool and an... CRISPRpedia IGI’s digital resources in English/Spanish ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....Campus and the University of California, Berkeley engineered an AAV variant that efficiently infects neurons...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....typically their own genome, but this step can be engineered to be anything you want delivered to your target...and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift mutations in genes of... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....routine, open-source software projects that actively engage with the community are particularly successful....hardware, this process is often much more involved. Challenges such as the provision of an accessible supply... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....Finally a set of wild type and codon optimized, full length and truncated versions of SARS-CoV-2 S (spike protein...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....developed a new calcium indicator, OCaMP. The challenge: Expanding the fluorescent toolbox Modern neuroscience...selecting a fluorophore matched to their excitation wavelength. OCaMP is well-suited for these lasers, with ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....asked questions about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There...There are a few important differences between engineered γ-retroviruses and lentiviruses. First, these viruses... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....expression of R-mAbs is that it can easily be engineered. Like humans, mice express multiple subclasses... Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog PostPublishedFeb. 11, 2021, 2:15 p.m....specific behaviors. Chemogenetics uses genetically engineered receptors to interact with small molecules to...Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....movement is that it provides interesting and unique challenges for intellectual property lawyers. While there...be, an interesting legal and professional challenge.Paratore: The promise there is the ability share the... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....SciComm by doing SciComm Writing had always been a strength of Susan’s, but she didn’t know if she liked science...finding that uninterrupted time to be creative is challenging,” says Susan. Another difficulty is that some... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....very pure sample. Because binding strength is proportional to the length of the tag, with longer tags you... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m....abundant in normal cells and are arise from a ssRNA engaging one strand of a dsDNA molecule; the 3-stranded..., but they also have a darker side. When ssRNA engages one strand of a dsDNA molecule, it does so at the... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....fragment, Fvs are often produced recombinantly and engineered to be joined by a linker peptide, resulting in...Tomisaki M, Kiuchi T, Takagi J, Watanabe N (2022) Engineered fast-dissociating antibody fragments for multiplexed... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....utilizes a gRNA which is photocaged and unable to engage a target sequence. Upon a brief light exposure ...Rosenberg, M., Hendel, A. Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....numbers of women participating in science and engineering. “Fix the Institutions” promotes gender equality...Gendered Innovations in Science, Health & Medicine, Engineering, and Environment—a project aimed at harnessing... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....time. Searching for literature My go-to search engines for finding scientific literature are Google Scholar...relevant. Harness the power of the Google search engine and sign up for Google Scholar alerts. Based on... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....Therefore they make excellent tools for genetic engineering. There are several different viral vectors to...our CRISPR reagents. Please check www.genome-engineering.org for the most up-to-date information. Check ... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful tools...Watabe-Uchida et al., 2012). They could also quantify the strength of the inputs by counting the monosynaptic inputs...