We narrowed to 530 results for: Erf

1. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ...the time spent by the scientists designing and performing the experiments. This cost varies hugely based...

2. Plasmids 101: The protein expression toolbox

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   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 ...

3. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism of...

4. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...the very nature of the way the experiments were performed, they did not see anything unique to male stem...

5. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...exciting applications, this system is an incredibly powerful tool for cloning complicated constructs in a single...

6. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...the selection marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...soCoChR podcast segment Optogenetic stimulation is a powerful tool used by neuroscientists to interrogate neuronal...

8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ... is part of a particular protein complex. Not performed in a native cell context. For AP-MS, cells are...

9. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...Platform and it suits our plasmid prepping needs perfectly. Working with Qiagen, we developed a protocol ...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...the experiment. Handling can however negatively interfere with certain tests and is not always recommended...

11. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type

    Blog Post

    Published

    Aug. 6, 2019, 12:55 p.m.

    ...your parental mice to generate F1 Once you’ve performed your first cross, congratulations! You’ve generated...

12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...researchers with useful and adaptable tools to perform basic and preclinical research. For scientists ...

13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...talk – to win the audience’s applause with your performance. When your institute opens its doors to the public...

14. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...methods flood the cell with protein, which can both interfere with a cell’s normal function and result in a ...

15. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas...

16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold. The ability of...

17. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...demethylation – tight binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9...

18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...the experiment by diluting your standard curve, performing a serial dilution of your experimental sample...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ... study the mechanisms of infection and how to interfere with it in a BSL-2 lab instead of a BSL-3 lab....

20. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment...