We narrowed to 776 results for: IRE;

...transfect’, such as primary cells. They can also be directly injected into hard-to-reach organs, such as the...like replication incompetent viral particles, are required. We will be covering these safety measures in ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

...catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....

...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...when encoding multiple guides. While two guides require two arrays for Cas9, two guides can be streamlined...

...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

...display-based directed evolution to create APEX2. They have now applied the same directed evolution technique... tagging proximal proteins within minutes, but requires H2O2, which can be toxic in living cells. BioID...

...activity, scientists can use GECIs to watch neurons fire under the microscope. To add another tool to our...developed calcium indicators spanning nearly the entire visible spectrum (Figure 1). The GCaMP series (... yellow and red fluorophores, allowing it to be paired with green, yellow, and/or red sensors in multicolor...

...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, for example,...

...because “it kinda sucked to work remotely. I was also tired of the contracting life where you do a little bit... was helping cover social media while the Hutch hired a full-time social media manager. “I usually start...magazine. Getting pulled in all of these different directions is sometimes at odds with the space needed to...

...RNA, a fast, strong protein denaturant would be required- something that broke down RNases before RNases... effective at isolating total RNA, this method requires a lot of time and depending on how many samples...without a kit!) As mentioned above, working with RNA requires keeping your samples cold until homogenization...

...Much of today's biological research requires a close examination of specific proteins within a system...tags, however, tended to be very large and often required the incorporation of a cleavage site and a post-purification...no cut or dried answer. Successful purification requires the tag to be accessible to the metal ions once...

...to read a formulaic fiction story that doesn’t require any detailed retention of information or formulation...something verbally. Sometimes a group was just really tired and needed a slower, simpler presentation than I... I had planned; other times students were fired up and enthusiastic and needed a faster presentation with...

...sufficient for certain patterning activities of entire tissue types or even organs. In frogs, ectopic ...electrical communication is briefly inhibited in the entire worm during regeneration, the worm creates two ... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...

...sex, the National Institutes of Health (NIH) now requires that sex be included as a biological variable ...variable in all NIH-funded research. Janine Clayton, Director of the Office of Research on Women’s Health, emphasizes...gender in science and technology and currently directs the EU/US Gendered Innovations in Science, Health...

...with multiple inserts or internal mutations that require more than two sequencing reactions. In the more...to ensure uniformity of the culture. Sequencing directly from the glycerol stock saves us the step of purifying... Each sequencing service will have different requirements for volume and concentration, and not all providers...

...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...coach usually will be a clear communicator and make direct suggestions for improvement and change. Work ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

...asymmetry in the core sequence gives the loxP site directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT...reversed.   Deletion: If the sites face in the same direction, the sequence between the loxP sites is excised...cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar...

...photon 2. Energy transfer from the excited donor directly to the acceptor fluorophore--think of it as a ...fusions varies with transfection efficiency and any unpaired fluorescent proteins can contribute additional...Check Out Fluorescent Protein Biosensors Browse the Entire Fluorescent Protein Collection ...

...effectively utilize the host cell’s inherent homology directed repair (HDR) process following the double strand...break and introducing the tag through homology directed repair To introduce the fluorescent tag to the...on the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining...

...viral infection by bacteriophages), or conjugation (direct transfer from one cell to another). Bacteria need...transfer genetic material to a recipient through direct contact. That’s conjugation! F factors can also...cloning. In addition, scientists have engineered entirely new types of plasmids without clear analogues ...

...Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived cells can alternatively...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...inflammatory diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR...