We narrowed to 666 results for: MAL

...enzyme. Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for...80% of the way down the gel will ensure you keep smaller bands from running off; however, you may need to...

...H2B (histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter...create GEmCherry2. The Denham lab made several other small changes creating 2 other versions but these did ...

...reverse tetracycline-controlled transactivator) is normally inactive and cannot bind the tetracycline response...Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters...

...Luciferase is the best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris... they emit or to optimize their expression in mammalian systems. Our luciferase collection page has a ...

... attain cell-specific expression in transgenic animals, where cre expression is restricted to a particular... which can then be exclusively activated by the small molecule clozapine N-oxide (CNO) (Krashes et al....

... replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and protomoting...preceded by GA or followed by TC) would be cut as normal. If not taken into account, this blocked site could...

... While commercial kits are available for making small point mutations, large deletions/insertions require...have some homology to each other; ~17 bases is optimal. After being treated with DpnI to digest away the...

...meeting new people. At large conferences, look for smaller events or meet-ups to connect with others more ...group sessions that match your interests. I favor small conferences where the attendees stay onsite and ...

...problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ...

...resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa.  Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently...

... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (...

...showed that Archon1 is functional in a wide range of animal models such as mice, C.elegans, and Zebrafish. ...Woolley lab developed a phage display technique using small scaffold proteins to identify novel binding proteins...

...becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database...

...want to program a cell to identify and target a malignant cell, then you’d need the ability to turn on genes...as sophisticated as those that living cells and animals make. That will be an outcome of genetic engineering...

...specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ...

...you. Once you input a sequence, SplitSet finds optimal fusion sites to split it into multiple fragments...antibiotic screening for selection, failed under normal cycling protocols, and required a 48-hour incubation...

..., and big hair. To what was perhaps a slightly smaller population, something else was also suddenly cool...distinguishes genes of some human cancers from their normal counterparts. Nature 301, 89–92. https://doi.org...

...endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic of the... fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence)...

...environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested...

...transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager...