We narrowed to 659 results for: MAL
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TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m....Luciferase is the best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris... they emit or to optimize their expression in mammalian systems. Our luciferase collection page has a ...
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How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m....meeting new people. At large conferences, look for smaller events or meet-ups to connect with others more ...group sessions that match your interests. I favor small conferences where the attendees stay onsite and ... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....becoming available to the community. This is no small task, however, at a repository that consistently...supplements), suggested sequencing primers, or mammalian selection markers. You don’t need a fancy database... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....resulting in two independent Fab fragments with small tails of the hinge region. Size: ~55 kDa. Fab -...handful of advantages over full antibodies. Their smaller size allows them to get into samples more efficiently... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m.... possibly because in vitro conditions did not optimally support growth of diverse species. These results...Carlotta, et al. "Metagenomic engineering of the mammalian gut microbiome in situ." Nature methods 16.2 (... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....showed that Archon1 is functional in a wide range of animal models such as mice, C.elegans, and Zebrafish. ...Woolley lab developed a phage display technique using small scaffold proteins to identify novel binding proteins... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....problem is, when it happens to other people, it’s normal and quickly forgotten, and when it happens to you...noises were just last week! Nerves It’s completely normal to be anxious, nervous, and/or apprehensive. I ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....want to program a cell to identify and target a malignant cell, then you’d need the ability to turn on genes...as sophisticated as those that living cells and animals make. That will be an outcome of genetic engineering... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell...6276558. 2. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....environmental plasmids, are all around us! A plasmid is a small piece of DNA that can replicate independently from... genetic parasites. Cryptic plasmids tend to be small and poorly studied, but some researchers have suggested... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....transitioned between different lab groups without having a formal interview process. Industry interviews, however...structured. I met with several individual and/or small groups, usually starting with the hiring manager... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....contains only the evolved TadA and is ~500 base-pair smaller than ABE8.x-d. This resulted altogether in 40...cell line for these versions they found that the smaller version, ABE8.x-m, showed comparable editing behavior... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....when the antibody is produced from a new set of animals), and wrong application (different experiments/...antibodies. A goldilocks antibody is highly specific (minimal binding to off-target molecules), reliable (wouldn... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m...., and big hair. To what was perhaps a slightly smaller population, something else was also suddenly cool...distinguishes genes of some human cancers from their normal counterparts. Nature 301, 89–92. https://doi.org... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs. Figure 2: Schematic of the... fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence)... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....ligase fusion protein identifies proximal and interacting proteins in mammalian cells. The Journal of cell ... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....persistent immunological reconstitution with a normally functioning T cell repertoire (1). In this example...icosahedral, non-enveloped virus that carries a small, approximately 4.7 kb single stranded genome. AAV... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m.... types of targets: primary cells, embryos, and animals. Nanoblades can also carry different types of CRISPR...delivery Learn about CRISPR delivery methods for mammalian cells Browse our CRISPR 101 blog post Resources... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....publish brief novel findings. These results are normally one figure and can be negative and/or even replicated...develop computational methods to better understand animal development (Kyoda et al., 2012). C. elegans plasmid... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... it with an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA...editing frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines...