We narrowed to 469 results for: NOL

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations...

2. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

   Type

   Blog Post

   Published

   Oct. 30, 2018, 1:04 p.m.

   ...pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009. 114: p. 185-99. PubMed PMID: 19343304....

3. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ... While T4 DNA Ligase, the mainstay of most biotechnological cloning efforts for over 50 years, prefers...

4. Fluorescent Tagging of Endogenous Genes with SapTrap

   Type

   Blog Post

   Published

   Oct. 12, 2017, 2:26 p.m.

   ...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   Published

   Sept. 12, 2017, 1:44 p.m.

   ...sequencing capabilities and Seqwell’s plexWell technology, we can now perform NGS at Addgene to sequence...

6. Fluorescent Proteins 101: Fluorescent Protein Timers

   Type

   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

7. PITChing MMEJ as an Alternative Route for Gene Editing

   Type

   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

8. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...

9. Genome-wide Screening Using CRISPR

   Type

   Blog Post

   Published

   Aug. 18, 2015, 2:30 p.m.

   ...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

10. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub...

11. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...new tools for plant science based around those technologies in the near future. Science on Twitter Addgene...

12. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization...

13. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...DNA fragments and chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins."...

14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type

    Blog Post

    Published

    July 16, 2020, 1:15 p.m.

    ...DNA-templated synthesis are four examples of technologies pioneered in his laboratory. Learn more here...

15. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...Indoor and Outdoor Air. Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT...

16. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    Published

    Dec. 22, 2022, 2:15 p.m.

    ...Cas9-mediated knock-ins in mammalian cells. J Biotechnol., 16(4): 387-390 (2020). 10.1016/j.jbiotec.2019.11.010...

17. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...-3.02523-X. Hofmann, C., Löser, P., Cichon, G., Arnold, W., Both, G. W., & Strauss, M. (1999). Ovine Adenovirus...

18. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...Transgenic Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi...

19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...genome editing in vitro and in vivo." Nature biotechnology 33.1 (2015): 73. PubMed PMID: 25357182. PubMed...

20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...widespread gene transfer to the adult brain. Nature biotechnology. 2016;34(2):204-209. PubMed PMID: 26829320. ...