We narrowed to 473 results for: NOL

1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ...s41596-020-0304-x Zimmermann I, Egloff P, Hutter CA, Arnold FM, Stohler P, Bocquet N, Hug MN, Huber S, Siegrist...

2. Polymerase Chain Reaction Overview and Applications

   Type

   Blog Post

   Published

   Aug. 10, 2021, 1:15 p.m.

   ...right. ddPCR uses a water-oil emulsion droplet technology that fractions the PCR reaction sample into approximately...

3. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   Published

   Nov. 5, 2024, 8:32 p.m.

   ...viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991...

4. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun. 13...

5. Antibodies 101: Conventional vs Spectral Flow Cytometry

   Type

   Blog Post

   Published

   Aug. 3, 2023, 1:15 p.m.

   ...one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations...

6. Hot Plasmids: Summer 2025

   Type

   Blog Post

   Published

   Aug. 28, 2025, 1:15 p.m.

   ...antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an antibody-like protein...

7. Hot Plasmids: Winter 2025

   Type

   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ...biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically...

8. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   Published

   Feb. 4, 2025, 2:15 p.m.

   ... development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily...

9. Of Myc and Men

   Type

   Blog Post

   Published

   Jan. 19, 2023, 2:15 p.m.

   ...mid-late 20th century with the advent of hybridoma technology and monoclonal antibodies. However, it was (and...

10. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...more difficult. I’ve learned that some of the technologies that helped us navigate a global pandemic can...

11. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...dominance over HDR in this species. As CRISPR technology continues to develop, it’s become clear that ...

12. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type

    Blog Post

    Published

    Sept. 24, 2015, 2:30 p.m.

    ...updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing purposes...

13. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...you'd like to brush up on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure...

14. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...selection and prevents autoimmunity. Holst et al (Nat Immunol. 2008 Jun;9(6):658-66. doi: 10.1038/ni.1611. Epub...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ...new tools for plant science based around those technologies in the near future. Science on Twitter Addgene...

16. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization...

17. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ...DNA fragments and chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins."...

18. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ...Transgenic Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi...

19. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type

    Blog Post

    Published

    Sept. 6, 2018, 12:02 p.m.

    ...genome editing in vitro and in vivo." Nature biotechnology 33.1 (2015): 73. PubMed PMID: 25357182. PubMed...

20. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type

    Blog Post

    Published

    Sept. 28, 2017, 2:01 p.m.

    ...widespread gene transfer to the adult brain. Nature biotechnology. 2016;34(2):204-209. PubMed PMID: 26829320. ...