We narrowed to 740 results for: NSI;

... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

...with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...

... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

...Foundation are used in Parkinson’s research.  The extensive resource from the Structural Genomics Consortium...Consortium enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium...

... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

...descriptions often fail to capture all of the responsibilities of a role, so it helps to see how people describe...path one you could see yourself taking? If so, consider setting up an informational interview with them...

...cupcakes, or maybe the lab member originally responsible for the deposit had already moved on to their...with journal articles. But we do encourage you to consider sharing them openly as soon as that preprint is...

... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

...for our quality control process because of its expansive feature library and useful tools. In this blog...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...

...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

...at his University of Wisconsin lab for a summer during my PhD and is responsible for my enduring love ...

...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...