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We narrowed to 729 results for: NSI;

Showing: 401 - 420 of 729 results

  1. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

  2. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...sequence variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus.... As you develop and confirm new gRNAs, please consider submitting their sequences (and plasmids!) so ...

  4. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  7. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound... experiments because  ultrasound devices are inexpensive, widely available, and have deep tissue penetration...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...researchers around the world. We take this responsibility seriously. Every decision we make, every project... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...generally follow the same pattern described above - a sensing domain induces changes in a fluorescent signal ...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables single molecule...SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...instead results in the breakage of the ester bonds responsible for the sugar backbone of the DNA helix or even...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA...

  13. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...a time. Just be sure there is a point person responsible for incorporating everyone’s suggestions into...timelines for experiments and manuscripts, who is responsible for which parts of the project, and, as mentioned...

  16. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Chance to do/learn new things, diversity of responsibilities, which might include training or teaching ...Nature Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own...

  17. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric gRNA...screened? A4: Before starting your experiment, consider co-transfecting with GFP. This allows you to sort...

  18. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...most applications. Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media...store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. Keep in ...
Showing: 401 - 420 of 729 results