We narrowed to 459 results for: PAC;

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

   Type

   Blog Post

   Published

   June 25, 2020, 1:15 p.m.

   ...while I've done that, my big takeaway has been the impact I can have on an organization beyond my job description...

2. Synthetic promoter AAVs for cell-type specific expression in retinal cells

   Type

   Blog Post

   Published

   March 12, 2020, 1:15 p.m.

   ... specific transcription factor binding sites interspaced with random sequences.   Strategy #4 (ProD): ...

3. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ...is clear borders around your columns, and good spacing between them so that they are easily distinguished...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...the other hand, lentiviral vectors are superior packaging vectors for larger genes - they can hold up to...

5. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...comparing dominant and recessive traits and their impact on diseases or treatments, but are not useful for...

6. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   Published

   Aug. 30, 2016, 2:30 p.m.

   ...field-based diagnostics, our approach could make an impact. Our motivation was underscored by the potential...

7. Plasmids 101: FLEx Vectors

   Type

   Blog Post

   Published

   April 28, 2016, 2:30 p.m.

   ...and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on Genetic Analyses...

8. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   Published

   March 31, 2016, 2:30 p.m.

   ...(A) signals in combination with certain viral packaging systems has been associated with reduced viral...

9. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

   Type

   Blog Post

   Published

   Jan. 21, 2015, 1:30 p.m.

   ...options for mutually beneficial solutions. If you package your challenges with your supervisor into learning...

10. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

11. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...

13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

14. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...and not valued which will undoubtedly negatively impact your work and your life. It’s up to you to decide...

15. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

16. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...strain Differences between E. coli host strains can impact plasmid yield. Check the bacterial strain to see...

17. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ... once a week to get recharged. The fun change of pace helped break up the negativity and allowed me to...

18. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...

19. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...

20. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...