We narrowed to 860 results for: PLE

...everyone was very approachable.     Fig. 1: Examples of DNA sequencing QC work    In addition to... core hours) at a time that works for you. For example, if I needed to go to an appointment at 3 PM, I...finished on time, there were no issues. Another example of helpful flexibility was the ability to work ...at Addgene. There is a clear effort to make sure people feel connected.    As I became accustomed to the...Northwestern University. She is one of several students completing their co-op at Addgene. This is her second contribution...

...response to a viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular... Erp, et al., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen... known as opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM...pathogen activates the immune system's classical complement pathway, starting a cascade of events that results.... PMID: 10742152. Dunkelberger, J., Song, WC. Complement and its role in innate and adaptive immune responses...

...medium containing the corresponding antibiotic. For example, if the DNA you’re inserting into the plasmid backbone...an essential amino acid), and a plasmid that complements the auxotrophy (i.e., synthesizes the critical... resistance spontaneously arises in yeast. For example, Markus Ralser’s lab created a yeast prototrophy...prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: Cells that have lost a specific... gene. SacB can be used similarly to CcdB. For example, in the gene expression plasmid pGTvL1-SGC from...

... last step will influence the stability of your sample over time and determine which experiments you can... impact the time you will be able to store your sample without having substantial degradation and which... DNA, it will limit what you can do with these samples. This is because divalent cations are essential...reaction, an effective possibility is to dilute your sample in water to reduce the EDTA concentration. Alternatively...temperature and longevity You should consider a couple of more things when you elute your DNA: where you...

...also contains multiple protocols and troubleshooting tips to make plasmid usage as simple and straightforward...for selection of plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene... topic suggestions for our Plasmids 101 series, please let us know in the comments. Note:Marcy Patrick...

...Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save any image... even include your own data in a ReFigure. For example, you might include a confirmatory image to show...visibility. For instance, long before a study with multiple patients was published, Zika researcher Christa...knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47...reach the ReFigure team at [email protected] Please support ReFigure by creating ReFigures and answering...

...energized by talking to people about science and that I love explaining things to people. When I started my...work better and because I really like talking to people about and communicating science. The path to science...useful and interesting than that will simply get people ordering plasmids. The need to keep content coming...plane back to Boston from Kentucky where I spent a couple of days talking to researchers about their work...

...biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted in..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...Resources page. Here are some highlights from the past couple months including several pooled libraries: Cas13X...found in the metagenome datasets from hypersaline samples, were engineered and adapted for RNAi experiments...

...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during...organism. Studying cell mobility within these complex environments has never been easy and, for many ...in thick specimens. See the video below for an example of two-photon intravital microscopy from PLoS Pathogens...scientists could easily track GFP expressing cells. For example, by harvesting lymph nodes at different times after...dynamics in lymphoid organs. In lymph nodes, for example, two-photon microscopy allowed scientists to better...time. Photoactivable fluorescent proteins, for example, have been successfully used to study B and T cell...

...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...another (e.g. green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek...state (“quenching”). The photoswitch can occur multiple times by activating with two distinct wavelengths...trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial...relies heavily on the same fluorophore being imaged multiple times in order to reconstruct an image. The best...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

...Binghamton University Cancer is one of the greatest examples of survival of the fittest. Cancer cells find ... use the blood stream to commute to and invade multiple organs, and most importantly escape the immune... also known to precede cancer development, for example, inflammatory bowel disease is associated with ...the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces to avoid...therapies must be explored. When attacking cancer, multiple immunotherapy targets can be utilized at the same...and suppression of tumor-aiding cytokines, for example, will more broadly activate the immune response...

... learning behavioral optogenetics in order to implement the technique in my lab at The Rockefeller University...physiology in order to directly show that, for example, the firing of a specific type of neuron in a specific...too but that’s what collaborators are for). For example, if you study neural pathways involved in addiction... proper controls are required and include, for example, confirmation of opsin expression in the intended...you may need to allow the virus to express. For example, if you inject into the ventral tegmental area ...22055387;  PMCID: PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct...

...more consistent, and more pleasant for everyone, which in turn encourages people to share more, since they...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

...efficient product of HMOs that could be used to supplement newborn diets and promote the formation of a ...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...researchers to engineer healthier microbiomes in people all over the world. Toward this end, she recently...training and wants to make her work understandable to people both inside and outside the scientific community...

...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

...make socially distanced work more creative. An example could be making lab meetings more interactive and...and brainstorm! Image from Joseph Mucira.   Implement new tools for creative interaction During Zoom...promote creative interaction. A great tool that a few people use is the built-in whiteboard, in which participants...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

...and therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s ...titers for successful transduction. In another example, neuronal gene expression by an anterograde AAV...visualize expression of fluorescent proteins, for example, as many viral particles must infect a cell to ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles to ...

...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...material pages associated with each item. There are multiple CRISPR tools available as lentivirus and we’ll...ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...the publications associated with these libraries. Please note, the pooled libraries we distribute in lentivirus...or are thinking about using our viral services. Please send any questions or comments to help@addgene....

... counter, lab timer, dilution calculator, and a couple of other nice features. This app also has a great...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information...can safely say that I still have the single and triple abbreviations of the amino acids memorized, but...researchers in the lab. If you have a favorite lab app, please share it in the comments section below! Many thanks...

...to the Cas9/tracrRNA complex. D) The mature crRNA is used to guide the Cas9 complex to the target DNA which...functions of the various CRISPR/Cas9 components. Please note that while this post is intended to provide...requirements of these different systems can vary (for example, xCas9 is a variant with increased PAM flexibiliy...remainder of this post, we’ll refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide...