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We narrowed to 792 results for: Por

Showing: 401 - 420 of 792 results

  1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...-hyg will be used to create gene deletions of important unknown regulatory regions in Pseudomonas and ... into a bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim...the user.   At Addgene we're happy to be able to support diverse groups of scientists working in a wide ...

  2. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...difficult during the pandemic era, with fewer opportunities for spontaneous conversations. What we are missing... minds. The final screen can also be saved and exported as a picture. I believe that showing something...fades away the longer we are on it – so it is important to take breaks. After 1 hour (or another designated...

  3. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ... for favors.  Don’t focus on networking with “important” or senior people. Focus on building relationships...times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There is a...children. When I had my baby, we became closer. She supported me and answered my questions and honestly, she...

  4. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine if the human interactors...includes a GFP construct as a control. For easier portability, all viral genes are flanked by EcoRI and BamHI...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks by torchlight...to all the optional sexual health sessions and reported the intel back to my shyer classmates. For my ...the community During my PhD, I’ve also found opportunities for more hands-on science communication. Between...

  6. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...facilitate a fast and agile MTA process. This is important to keep up with the number of requests and deposits...for both research and commercial purposes. It’s important to note that it’s the depositing institute that...signatures from two different institutes. Our online portal automates a lot of the process, from notifications...

  7. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...scientists. In addition to maintaining a laboratory to support both individual and community science projects,...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept... involvement and provide all our members the opportunity to do molecular biology, we’ve developed community...

  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...when performing amplifications, and we use electroporation to ensure a good transformation efficiency....library, Addgene usually does four to eight electroporation reactions using a total of ~400ng of library...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... Lab Archives by LabArchives The single, most important part of lab life is the lab notebook. Lab Archives...always comes first with research! It is always important to know what kind of reagents you work with in...iGreenEarth AMINO is a very simple app that packs important biological information, amino acid abbreviations...

  10. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...detected signal and therefore high brightness is an important feature of a fluorophore. The key question is ...However, the theoretical brightness ignores several important experimental conditions that are related to the...

  11. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...histones and chromatin architecture to every diagram portraying some aspect of eukaryotic DNA would become busy...plasmids available from Addgene Many articles reporting chromatin-related research describe the production...Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small...

  12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...producing long unspecific RNA fragments that are incorporated into the dsRNA necessary for RNAi. The Vellai...adjacent to the T7 promoters to prevent the incorporation of non-specific nucleotides into the dsRNA leading...enzyme and a small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific...

  13. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...as well. In a screen, you aren’t killing off a portion of the cells like in a selection. Instead, all ... genome, and scientists have found that when a portion of the gene is deleted, it creates a non-functional...Complementing this mutation by expressing this deleted portion, for example on a plasmid, creates a functional...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    .... The advantages of optogenetics lie in the spatiotemporal control offered by the system that allows for... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... in sample treatment across methods can affect reported titer by either method so to ensure equal animal...AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction. While...marmosets each. Brain potency for each individual is reported in Figure 4B (see left for image and link for ...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ... enough to enter the nucleus through the nuclear pore complex. This feature may be useful for the transduction... expression of transgenes via increased nuclear export. LTR Subcomponents: -U3 -R -U5 LTR; Long...

  17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ...is to understand the function of the non-coding portion of the genome. Mendenhall and Richard Myers of ...efficiency of inducing double strand breaks and more opportunity for homologous recombination. Before CRISPR, ...versions of this out there. Addgene: Why was it important to you to make these plasmids available to other...

  18. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop...knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown that the SAM system is robust...size of the backbone, transformations require electroporation. References & Further Reading: For more ...

  19. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...potential conflicts that waste money and time. Most importantly, “the company does not become real until someone...Synthetic Biology? Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup...

  20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...with their planned raises, some are taking the opportunity of the lapsed mandate (or are being compelled... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...Careers Resources on Addgene.org Find Career Opportunities at Addgene  ...
Showing: 401 - 420 of 792 results