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We narrowed to 837 results for: RING

Showing: 401 - 420 of 837 results

  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...risk for the project by initially designing and ordering several dozen versions of the toehold switches...successful sensors to draw on as our assays grew more stringent, and it avoided any days of lost productivity ... virus genome with Addgene to facilitate rapid sharing of these tools with the Zika virus research community...product development, the regulatory process, and manufacturing so that we can get the diagnostics into the ...

  2. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...shortcomings. Cpf1’s preferred PAM is 5’-TTN, differing from that of Cas9 (3’-NGG) in both distribution...clinical applications require HDR over NHEJ, so comparing HDR efficiency across CRISPR nucleases and NgAgo...insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two processes will help... Mary Gearing...

  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ... subtypes, thanks to viral serotype specificity during delivery (more on this later). Chemigenetic sensors... at the single neuron level in vitro. In vivo monitoring has also been achieved with these sensors, but...their fluorescence, which ultimately led to the engineering of the second type of these GEVIs: a FP-fused...component, the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically...

  4. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...not recognize the mouse or chicken homologs. By comparing the homologous sequences in these species, they...making the antibody more amenable to protein engineering. As a result, you can now find Myc1-9E10 in a...published a paper showing that the amino acids neighboring the Myc epitope influence 9E10’s affinity for...highly variable epitope recognition dependent on neighboring sequence context. Sci Signal 13. https://doi....

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...re a presentation veteran, but have difficulty answering questions. Or perhaps you’re simply attending,...snacks to keep you energized throughout the session, bring extra clips to hold up your poster, and pack a few...seems captivated. Expertly navigate questions Answering questions is arguably the most difficult part ...very interested in his/her work, and would enjoy hearing the highlights so that you can appreciate the impact...

  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... Manuel Gonçalves; made with BioRender.   Delivering gRNA and Cas9 using adenoviral vectors As compared...published in Nature Methods the same year that delivering RNA-guided nucleases or TALENs together with ...the lab of Andrea Ventura at the Memorial Sloan Kettering Cancer Center did to create a model of Eml4–Alk-driven...Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering of oncogenic chromosomal rearrangements with ...

  7. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...mine you didn’t think much about management skills during your scientific training. I never thought about...encourage questions. Unless you’re delegating a recurring task to a seasoned report, there should be questions...cases reports are not aware of issues until you bring them up. I wouldn’t go as far as saying that your...

  8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...activity – it only requires the presence of oxygen during maturation. This meant that the protein could be...study of dynamic, physiological processes. Re-engineering GFP to increase its range of color and application...its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis in order...us how! And come back next week when we'll be featuring a guest blog post from Gal Haimovich of greenfluorescentblog...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...eukaryotic transcriptional activation and cell biology. During this period, proteins were successfully expressed... own, but, if the two POI’s interact, this will bring the POI-TA fusion (commonly referred to as the “.... Lowered expression levels will increase the stringency of your binding. And most importantly: Independently...Ptashne, M. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor....

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation process, ...never receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria...while amp only affects the cell wall synthesis step during cell division. This is nifty because the recovery...it will induce mutations in the host/plasmid DNA during selection if it’s not completely bound up. While...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...find out! Homology-directed repair and genome engineering The easiest way to make a knock-in cell line ...built-in repair pathway cells already have for repairing DNA double strand breaks – (HR). HR is the dominant..., H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response to.... A. Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery....

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...steps and outlined the considerations to be made during each step. Now that you know the basic principles...harsh - lipids and soluble proteins can be lost during fixation with these chemicals, which impacts sample...and attention.   * There are other methods for recovering epitope availability following cross-linking ...Immunofluorescence Staining. Methods in Molecular Biology. Springer New York, pp 299–311. https://doi.org/10.1007/...

  13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...minigenome system Similar to other RNA viruses, EBOV brings its own RNA-dependent RNA polymerase into the infected...partially control expression levels of each gene by altering the amount transfected (see Technical Tips). Minigenome...changing the minigenome cis-acting sequences and monitoring effects on genome replication and transcription...strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed PMID:15298166...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...neuronal loop which is activated and strengthened during memory formation and memory retrieval. In neuronal...recordings. The origins of these methods are naturally occurring viruses that infect, persist, and migrate within...modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including pseudorabies...plasmids at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely...AAV1 – 9, with most of these serotypes capable of entering neurons (see table 1)(Choi et al., 2005; Taymans...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...for the entire translation process. For example, during high growth rate conditions, E. coli preferentially...biosynthetic enzymes may be preferentially charged during amino acid starvation thus resulting in higher ...getting your genes of interest codon optimized by ordering plasmids containing them from Addgene. If a plasmid...that will generally segregate with the cell pellet during purification procedures. Even if you produce a ...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ... not all, of these new experimental species. Discovering ways to culture, propagate, transform, store,...systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed to expand the number..., in general, these labs all include materials sharing in their dissemination plans and in particular,...to mate with and if she decides to raise their offspring or leave them to their own devices. Chytrid fungi...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...phage-assisted continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,... increase the utility of this technique.  By engineering a light-sensitive LOV domain into the TurboID...mass spectrometry workflow identifies proteins bearing both labels (which had therefore been trafficked...

  18. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is thrilled to partner...methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird, 2019) and...neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...have shown exciting and promising results for repairing mutations in models of neurological disease. Klaus...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...but did not consider them for myself. I started picturing myself in a technical support role only when I...there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house testing...are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance the OD signals...with other departments such as research and manufacturing, quality assurance, product management, marketing...

  20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ... the cut site were not efficiently incorporated during HDR. We decided to systematically characterize ...References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." Science 339.6121 (...Mali, Prashant, et al. "RNA-guided human genome engineering via Cas9." Science 339.6121 (2013): 823-826. ...Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org...
Showing: 401 - 420 of 837 results