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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 847 results for: STI;

Showing: 401 - 420 of 847 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Restriction digest screening for ITR deletion is still necessary. To save time and money, I like to miniprep...Production protocol, I take a lazier approach for harvesting my AAV. I skip the PEG precipitation of the HEK293...PEG solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...

  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...kinds of CRISPR tools people were depositing and requesting each year (Figure 2). We did a similar analysis...2012 to 2023; data are smoothed using locally estimated scatterplot smoothing. (C) Number of requests ...continue to develop and share new tools, while our requesting labs continue to request and use new tools. Because... each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea; CH=Switzerland...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...have specific web sites for postdoc listings, so the method of posting varies between companies. Like most...that they were having a positive experience, I was still incredibly nervous about leaving academia. A lot...seen listed as a requirement on some industry job postings. Also, if you work in industry for any length ...

  5. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... vector (deposited by David Root of the Broad Institute) has consistently been one of the repository’s...have used the protocol for this plasmid, and if it still being used. After learning of it's continued popularity...is the creation of ZappyLabs. Currently in beta testing, the software aims to be a life science protocol...bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me – I didn’t get...

  6. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ... the “Align two or more sequences” option to drastically reduce waiting time for search results. Optimizing...with the "Align two or more sequences" option and pasting your expected protein sequence into the Subject...webservices/#pairwise_sequence_alignment_psa Happy BLASTing! Do you have any tips for using BLAST to confirm...plasmid sequencing results or comments on our suggestions? Share your thoughts here to help other labs ...

  7. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...editor, the lab started with the Cas13 scaffold, testing a whopping 21 Cas13a orthologs, 15 Cas13b orthologs... the plasmids for the RESCUE RNA editors here! Testing and improving RNA editing from the Cas13b REPAIR...knockdown is more precise than shRNA knockdown, it still displays substantial off-target activity. By mutating...Addgene. East-Seletsky, Alexandra, et al. "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...is currently investigating the molecular biology of synaptic transmission and plasticity.     Javier García-Nafría...co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the PCR mix to remove ... García-Nafría is an investigator scientist at the MRC Laboratory of Molecular Biology. He is interested...

  9. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...labs of David Root and John Doench at the Broad Institute have developed human genome-wide CRISPRko (knock...lowly expressed genes or genes that are more drastically affected by gene activation rather than depletion...resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...2020). Harvest and purification At its core, harvesting recombinant antibodies consists of separating...Created with biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are... the efficacy of our antibodies through stress testing. An aliquot of our recombinant antibodies is stored...assays showed that the majority of antibodies are still equally effective compared to those aliquots frozen...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    .... To kick off a knock-in experiment the first questions are where, which, and what. Identify where in ...like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done through...HDR edit, you can try one or more of the below suggestions. Disable the other repair mechanisms Mammalian...! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote HDR by generating...

  12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...clicking the ‘Search’ button a new content-aware suggestion dropdown list will let you jump directly to the...types of searches including depositing labs and institutions. For example, search for “Melina Fan” and you... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your...plasmids specifically designed for this purpose: Still stumped? Ask an Addgenie The newly launched search...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...Janelia Research Campus, Howard Hughes Medical Institute. Chemistry is dead, long live chemistry! The discovery.... These impressive advances lead to an obvious question: In this age of GFP and its ilk, why should cell...Janelia Research Campus, Howard Hughes Medical Institute. He would rather be in the lab making new fluorophores...PubMed PMID: 26575238. 5. Crivat, Georgeta, and Justin W. Taraska. "Imaging proteins inside cells with...

  15. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ... It can be so overwhelming that you might just stick with the protocol your labmate handed you — after... contain any denaturing agents. However, you’ll still have to lyse your proteins. For this post, we are...stronger reducing agent than BME and has a less… distinctive smell. BME smells like rotting eggs but has a...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that... may only have access to one or another. Most institutes have moved towards CCD cameras over the past ...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...interneurons across species and will undoubtedly constitute a valuable tool for the research community. You...CaMKII and RhoA during induction of structural plasticity in single dendritic spines. The set of CyRFP ...variants to develop five new base editors with distinct PAM sequences, expanding the number of available...xylose-inducible dCas9 paired with a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each...to premature aging and cancer. The Denchi lab investigated an aspect of telomere length regulation called...

  17. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...cardio-respiratory dysfunction, distinctive hand movements, and show autistic-like behaviors.  The role of...animal models results in rapid symptom onset, suggesting that MECP2 is also essential after development...). Beam Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... delve deeper and look specifically at the characteristics and capabilities an ELN needs to facilitate...Goettingen, and several other large research institutions. The following graphic depicts the ways in which...banks and sequencing files is likely stored in institutional and lab servers specifically designed to hold...illustrated in this video, and to data stored on institutional and lab servers. Connectivity to other tools...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... PMC5726555. 4. Xie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing... a single base. If you heard about CRISPR, one question probably comes to mind: can CRISPR be applied ...Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin ...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...there are still many more features to consider when designing a full gene. A non-exhaustive list includes...be many possible ways to encode them and what constitutes the “appropriate” codon is not necessarily obvious...PubMed Central PMCID: PMC2970903. 6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary conservation...
Showing: 401 - 420 of 847 results