We narrowed to 813 results for: TIM;

...antibodies that have almost certainly already been optimized for your application. Depending on the tag, directly...of epitope tags exist – all with individual optimizations and specific monoclonal antibodies. Below we... acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein’s...

...undergraduate degree (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience...findings of brain science, my curiosity did not ultimately translate to a desire to do the research itself...percolate within a new intellectual environment. Over time, this interest came to incorporate other facets ...

...communication research.   This summer, we’re making time to talk about my favorite thing - science communication...knowledge than average and are more likely to spend time engaging with science communication efforts.  Scientific...about science communication itself! See you next time!  Additional resources on the Addgene blog 10 ...

...this; however, you should reduce your incubation time on ice to 5 minutes (incubating the full 30 minutes...group! You may want to include extra extension time after the last cycle of PCR to make sure that the... is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies have been...

...launched in the early spring of 2015 and since that time have received about 3 project proposals per month...making connections and finding mentors -- well, it's time you call upon these people for guidance.  Before... evolve and respond to feedback throughout its lifetime.  In this way, you will always improve and give...

...University of Cambridge and knew it was time to leave. I really enjoyed my time in the lab, my colleagues and what...choices were mostly by chance. Luckily, around the time I decided to look for work outside of academia, ...

... job. EAs shouldn't be viewed as an unproductive time spent away from the bench, but as an opportunity... conferences and seminars, as this is a valuable time to network Attend informational interviews to ...required qualifications and skill sets Taking the time to identify and research 2 – 3 potential professions...

...it required only about 15 minutes of my hands on time and a total of about 3 hours. It was robust, capable...to learn something new without spending a lot of time developing and making space for the techniques ourselves... ourselves. As you probably have limited time and space in your own lab, you should give similar workshops...

... gRNA library, and we thought it would be a good time to talk about the amplification and verification...in the library. Bacterial growth must also be optimized to maintain library diversity. If the various ...seemingly straightforward – you need to count how many times each of the library components is found in the read...

...transparent, so it’s easy to watch development in real time.  In both cases, dL5** was tagged with mCerulean3...They found that KillerRed required longer exposure times (5 minutes vs 10 seconds) to achieve the same level...experienced minor bleaching after longer exposure times. Additionally, KillerRed requires mitochondrial ...

...as the relatively low cost and quick turn-around time of Sanger sequencing have historically matched the...The cost of these normalization steps, measured in time and consumables, can be daunting for large numbers... have worked with Addgene to develop a highly optimized bioinformatics pipeline that will return high-...

...Group (QCRG) to do just this.  In under a month’s time, we’ve published an article on BioRxiv describing...!) Attacking coronavirus, one FedEx package at a time Since the publication of our paper, our group has...fulfillment manually in a shared spreadsheet and it takes time away from our research. For all these reasons, the...

...easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation ... K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for genome engineering...cerevisiae and S. pombe - aren’t going anywhere anytime soon, and for good reason! However, there are other...

... DAP array, short for “drive-and-process.” To optimize DAP use, they developed a generalizable method...sequences must be avoided, different tRNAs with optimized 5’ leader sequences can be used together in DAP... Reporters to Monitor and Enrich Editing in Real Time Prime Editing: Adding Precision and Flexibility ...

...iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the...generations of iGluSnFR had limitations: they were not optimal for experiments requiring high signal to noise ...Tools References Marvin, Jonathan S., et al. “An optimized fluorescent probe for visualizing glutamate neurotransmission...

... its interaction with the degron sequence and ultimately its degradation. While other eukaryotes don’t...transcription factors tethered to dCas9, require long time scales with constitutively expressed proteins that...dynamic nature of genomic looping due to it’s briefer time scale and its inducible and reversible nature. ...

... was to be revisited at meetings when sufficient time, additional public fora for data review, and more...consensus guidelines, but there was never a specific timeline for this plan—or any action to follow it. And ... 2018 saga of “what’s happening in genetics this time?” She lives with her partner (in business and life...

...emails, chats, pictures, and apps. Throughout my time as a graduate student, I have relied more and more...requires cell culture and include a counter, lab timer, dilution calculator, and a couple of other nice... Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence and the NEB...

...microbiology class my sophomore year and it was the first time that I was in a lab class where we had an assignment... me away….  I have to come here, I need to spend time here, this is an incredible place.” Doench has spent...CRISPR, just the results are so much cleaner. The time frame from doing your primary screen to knowing ...

... negative selection in plasmid cloning.  But oftentimes, a selection strategy is not enough on its own...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...