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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 743 results for: TRI

Showing: 401 - 420 of 743 results

  1. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ... experiments to the PIs. Remote meetings can be tricky, and it takes extra effort to make sure everyone...everyone in the meeting has an equal opportunity to contribute. Using video conferencing tools like Zoom or ...

  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...    sdAbs in research These advantages have contributed to sdAbs becoming valuable tools for diverse ... approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics...

  5. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer...

  6. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...for sale, and photos of the Agar Art competition entries were displayed in a gallery on the second floor... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral...

  9. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...one step involves boiling the glass powder in 50% nitric acid, but I never let that stop me. I only had ...glass slide using only surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t...

  10. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...to the antibiotic that remains intact. Tips and tricks from the bench Use fresh stocks. Most antibiotics... Marcy Patrick...

  12. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

  14. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test...determine if your sample has recombined. Choose a restriction enzyme that will give you different band patterns...

  15. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    Published
    Jan. 24, 2019, 2:24 p.m.
    ...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

  17. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...don’t use SnapGene yet, you can download a free trial. If you notice that a feature is missing from our...

  18. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

  19. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...
Showing: 401 - 420 of 743 results