We narrowed to 484 results for: Vit

1. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...functions. Most individual parts have characterized activities with predictable behaviors. While a gene is the...

2. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...underlying causes of this unreliability: cross-reactivity (binds to non-target proteins), variability (...

3. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...quantitative readout of the interaction in vivo or in vitro, and can be used for measuring protein expression...

4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

5. 5 Tips for Troubleshooting Viral Transductions

   Type

   Blog Post

   Published

   Aug. 11, 2016, 2:23 p.m.

   ...given viral stock will give you a sense of the infectivity of your viral stock, save you time, and allow...

6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ...Canton Nucleic Acids Forum (CNAF) 2015 conference invited me to attend and speak about Addgene’s experience...

7. How-to: 5 Steps to a Great Panel Discussion

   Type

   Blog Post

   Published

   Oct. 22, 2014, 1:57 p.m.

   ...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

8. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

9. Plasmids 101: Protein Expression

   Type

   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...cell-free expression systems, proteins are assembled in vitro using purified components of the transcription and...

10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type

    Blog Post

    Published

    Sept. 12, 2017, 1:44 p.m.

    ...contamination is a good start, but to determine the sensitivity of our method we wanted to see how low we could...

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    Published

    July 13, 2017, 2:30 p.m.

    ...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

12. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...useful for your next localization experiment and invite you to make any fluorescent protein constructs ...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...for trimethoprim, there would be no changes in sensitivity. Pooled knockdowns are also possible with Mobile-CRISPRi...

14. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type

    Blog Post

    Published

    Oct. 23, 2018, 11:49 a.m.

    ...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

15. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ... receiving applications, universities typically invite a select group of students to continue the application...

16. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...Jung, D., Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based...

18. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...of new tools, illustrated in Figure 2, and the longevity of tools seen in Figure 3, indicates that the ...

19. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

20. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ... Doench CRISPR-Cas9: Tips for Optimizing sgRNA Activity by John Doench All CRISPR Posts  ...