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TypeBlog PostPublishedOct. 31, 2019, 1:06 p.m....gills. It attaches there until they mature and changes sex from male to female. Then, it detaches from... Jennifer Tsang...
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Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m.... desserts. We enjoyed a video highlighting the changes at Addgene over the last 10 years as well as the...fruition. The concept, though relatively simple, changed the course of scientific research. What was once... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m....transcriptional activation and genome editing Yu Wang's lab developed drug inducible CRISPR/Cas9 systems... Jennifer Tsang... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog PostPublishedJune 26, 2017, 1:31 p.m....Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange) scientists largely cluster by discipline, suggesting... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m....proteins that would fall within that near-infrared range, and that’s exactly what iRFPs achieved. Those proteins... proteins that operate within the 750 to 800 nm range, and his team is also looking in the direction of... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....conformational change in the GPCR, leading to the activation of the Gα subunit. The activated Gα exchanges bound... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....contamination. Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...for the sugar backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog PostPublishedAug. 22, 2019, 12:43 p.m....used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows... Jennifer Tsang... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...29853555. PubMed Central PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog PostPublishedFeb. 14, 2019, 2:35 p.m....are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid pages We’ve given...RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue for temporary... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog PostPublishedNov. 1, 2017, 2:04 p.m....matter expert in the educational sphere, it can range from researching an area, to writing an introduction...lab classes. However, their opinions are often changed after seeing a kit for themselves. “You open up... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m....Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...Data-optimized Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog PostPublishedJune 29, 2023, 7 p.m.... suggest changes to our social media strategy. We ended up implementing some of these changes and seeing... -
Build Your CRISPR Vocabulary
TypeBlog PostPublishedJuly 2, 2024, 1:15 p.m....tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural... -
We're Updating Our Hold for Publication Policy
TypeBlog PostPublishedJune 24, 2025, 1:15 p.m....are distributed. Next month, we are making two changes to the policy that will make the process a bit ...updating our Hold for Publication policy with two changes that will help us ensure that plasmids don’t get... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5... -
Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...