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We narrowed to 811 results for: cha

Showing: 401 - 420 of 811 results

  1. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature...manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

  2. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ... for giving feedback Use formal and informal mechanisms for feedback (more on this below). Make sure...positive outcome if the employee sees that real change is possible in your eyes so their confidence is...feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening way...

  3. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... of freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities...it, we are special. Below are some special characteristics of scientists in the work place as described...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

  4. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates the ... and what can be left out, which can be a big challenge!  The resolution Throughout your conflict, you... and driver of your story gives your audience a chance to get curious and invested in the research itself...

  5. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...independently” and “the power or opportunity to act or take charge before others do”. Candidates who have “started...including those that find events stressful. Video chatting makes it pretty easy to make 30 minute appointments...being able to just look at a paragraph and do a character count in your head.  Here are some sites that ...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few...AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV ...AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype...

  7. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...cancer-specific research. Membrane receptors and ion channels are often exogenously expressed and studied in...proteins and its relatively low expression of native channels.  No matter what your research question, HEK-...research interests include receptor biology, ion channels, or involve human protein preparations with PTMs...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...for the measurement of autophagic flux, or the changes in autophagic activity, and are a more reliable...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker for measuring...functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic environments...

  10. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...nice to have, not a need to have Must be ready to change projects frequently with little notice or choice...someone else who can do better, no grant writing Job changes can happen regularly (every 4-6 years is common... create interpersonal issues (one bad apple can change entire culture) Specific requirements for hiring...

  11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...against invading DNA. In 2014, Swarts et al. characterized Argonaute from Thermus thermophilus (TtAgo),...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

  12. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...you don’t take this past the chatting stage you will rarely make any change in your skill sets or activate...formality to mentoring Just getting together and chatting with another person about their career path is...

  14. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety... fused base editors (nCas9-BE), as Cas9 is mechanistically more favored for efficient base editing. They... To do so, they harnessed the tRNA processing mechanism, which is conserved in all living organisms and...

  15. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from... from the inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated...response than its predecessors. The detailed characterization of the new variants is available here. Upgrades...

  16. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis Quantifying...fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis....

  17. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ... Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...their peroxisomes will proliferate upon a media change into methanol and peroxisomal autophagy can be ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Susanna Stroik The Doudna lab’s recent work characterized a new tool for RNA targeting, with a leg up ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...denaturing conditions, high temperatures, and other chaotropic conditions better than most FPs. The group also...

  19. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...and accurate sequencing (“reading”) of DNA, have changed our collective thinking about the feasible size...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  20. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...throughput functional characterization of specific edits to the genome. Such characterization can be done in ...The RNAi process had just been discovered, the mechanism was barely understood.” Doench’s graduate work...
Showing: 401 - 420 of 811 results