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We narrowed to 811 results for: lars

Showing: 401 - 420 of 811 results

  1. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...the cell, and potentially spread out over an even larger area, thus necessitating even higher retrograde...methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-...Adeno-associated Virus Transduction and Integration. Molecular therapy : the journal of the American Society ...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We ...at the MIT Sloan School of Management. She is particularly interested in exploring how innovative tools...

  3. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding... realized that these mutations clustered in a particular region of the R-body. Further mutatgenesis of...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...

  4. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...

  5. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...contain all fluorescent proteins in common use (particularly not the most recently published ones), they ...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...

  6. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

  7. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

  8. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

  9. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...biobuilder.org) or by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...

  10. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos were designed with...

  11. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...

  12. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics...Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

  14. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

  17. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

  18. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

  19. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...method, but can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with...leveraged the power of Golden Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo...101: Multiplex Expression of gRNAs Choosing a Molecular Cloning Technique  ...

  20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...
Showing: 401 - 420 of 811 results