We narrowed to 440 results for: pes;

1. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...increase cloning efficiency to >99%. Universality All types of DNA fragments may be cloned: PCR fragments, cDNA...

2. Antibiotic Resistance: An Old Solution but a New Problem

   Type

   Blog Post

   Published

   Nov. 10, 2016, 3:30 p.m.

   ...resistant to three or more different classes, or types, of drugs used to treat them. Very often, this is...

3. Plasmids 101: Stringent Regulation of Replication

   Type

   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ...machinery for elongation and termination. These types of plasmids tend to be high copy number. Here, we...

4. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ... in vivo studies. Let’s take a look at the many types of plasmid addiction systems and their applications...

5. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue), which target...

6. 5 Reasons to Use Reddit for Science Communication

   Type

   Blog Post

   Published

   Oct. 20, 2016, 2:30 p.m.

   ...use it for everything from receipts to blogs to recipes. I am not paid to say this, but I really don’t ...

7. CRISPR-based Genome Editing Systems For Editing in E. coli

   Type

   Blog Post

   Published

   April 13, 2021, 1:15 p.m.

   ... biochemical mechanisms behind their distinct phenotypes and to design a new generation of engineered ...

8. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ... due to their low immunogenicity and range of serotypes allowing preferential infection of certain tissues...

9. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ... brain cells. Find this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA...

10. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ...which could limit their use across different cell types and systems. To introduce flexibility, the Dow lab...

11. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...could accurately distinguish between two similar types of HPV, HPV16 and HPV18, from human cells, at atomolar...

12. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    Published

    June 28, 2016, 10:59 a.m.

    ...determine the pros and cons of using many different types of gRNA design software. When you are happy with...

13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...replication machinery of Kaposi’s sarcoma-associated herpesvirus.       References 1. Snapp, Erik. "Design and...

14. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...of c-fos amongst animals that receive different types of stimulation may help to refine and optimize your...

15. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ...be discussed later). In addition to the basic genotypes outlined below, certain specialized strains are...

16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...ScFvs can also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune...

17. Five Popular Model Organisms

    Type

    Blog Post

    Published

    April 11, 2019, 12:39 p.m.

    ... diseases. Diseases in mice show many similar phenotypes to human diseases and have successfully helped...

18. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...using a biotin labelled dUTP. There are multiple types of biotin labels that can be added to an oligonucleotide...

19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...of choice for gene delivery to a variety of cell types, now you know that AAVs can provide a powerful boost...

20. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ...Disadvantages of this method include favoring of certain types of mutations, difficulty controlling the rate of...